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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated PRM1 Polyclonal antibody

PRM1 Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-15697

Synonyms

Cysteine rich protamine, P1, PRM1, protamine 1, Sperm protamine P1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CoraLite® Plus 488-conjugated PRM1 Polyclonal anti (CL488-15697)

    IF Staining of mouse testis using CL488-15697

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using CoraLite® Plus 488 PRM1 antibody (CL488-15697) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-15697 targets PRM1 in IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRM1 fusion protein Ag8323 相同性解析による交差性が予測される生物種
Full Name PRM1
Calculated molecular weight 51 aa, 6 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC003673
Gene symbol PRM1
Gene ID (NCBI) 5619
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen Affinity Purified
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRM1 is a 51 amino acid protein. PRM1 substitute for histones in the chromatin of sperm during the haploid phase of spermatogenesis. They compact sperm DNA into a highly condensed, stable and inactive complex. PRM1 is expressed in testis. The calculated molecular weight of PRM1 is 9 kDa, but Phosphorylated protein is about 20 kDa. (PMID: 24818823 )

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 PRM1 antibody CL488-15697Download protocol
Standard Protocols
Click here to view our Standard Protocols