PRM2 Polyclonal antibody
PRM2 Polyclonal Antibody for WB, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (1)
Applications
WB, IHC, ELISA, IF
Conjugate
Unconjugated
Cat no : 14500-1-AP
Synonyms
Validation Data Gallery
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Tested Applications
| Positive WB detected in | human testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 6 publications below |
| IHC | See 1 publications below |
| IF | See 4 publications below |
Product Information
14500-1-AP targets PRM2 in WB, IHC, ELISA, IF applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PRM2 fusion protein Ag5952 相同性解析による交差性が予測される生物種 |
| Full Name | protamine 2 |
| Calculated molecular weight | 13 kDa |
| Observed molecular weight | 10-25 kDa |
| GenBank accession number | BC066338 |
| Gene symbol | PRM2 |
| Gene ID (NCBI) | 5620 |
| RRID | AB_2721024 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PRM2 (Protamine-2) is the main DNA-binding protein in the sperm nucleus, consisting of a C-terminal mature PRM2 (mPRM2) domain and an N-terminal lytic PRM2 (cPRM2) domain, which is lyzed sequentially after binding to DNA. PRM2 plays an important role in spermatogenesis and development. PRM2 is specifically expressed in sperm and localized in the sperm nucleus.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PRM2 antibody 14500-1-AP | Download protocol |
| IHC protocol for PRM2 antibody 14500-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Mol Cell Chromodomain Protein CDYL Acts as a Crotonyl-CoA Hydratase to Regulate Histone Crotonylation and Spermatogenesis. | ||
J Hazard Mater Multi-omics analyses reveal the mechanisms of Arsenic-induced male reproductive toxicity in mice. | ||
Environ Pollut Silica nanoparticles induce spermatogenesis disorders via L3MBTL2-DNA damage-p53 apoptosis and RNF8-ubH2A/ubH2B pathway in mice. | ||
Environ Pollut Silica nanoparticles inhibiting the differentiation of round spermatid and chromatin remodeling of haploid period via MIWI in mice. | ||
Hum Reprod Posttranslational lysine 2-hydroxyisobutyrylation of human sperm tail proteins affects motility. |
