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PRMT1 Polyclonal antibody

Name: PRMT1 Polyclonal antibody
Brand: Proteintech
SKU: 11279-1-AP
Rating: 5.00 (1 reviews)

PRMT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugate

Unconjugated

Publications

Reviews (1)

Cat no : 11279-1-AP

Synonyms

Interferon receptor 1-bound protein 4, HRMT1L2, Histone-arginine N-methyltransferase PRMT1, HCP1, EC:2.1.1.319

Validation Data Gallery

Filter:

AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 11279-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 11279-1-AP
WB result of PRMT1 antibody (11279-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PRMT1 transfected A549 cells.
WB analysis using 11279-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 11279-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 11279-1-AP (PRMT1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IP experiment of A549 using 11279-1-AP
IP result of anti-PRMT1 (IP:11279-1-AP, 4ug; Detection:11279-1-AP 1:2000) with A549 cells lysate 920 ug.
IHC staining of human colon using 11279-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11279-1-AP (PRMT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon using 11279-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 11279-1-AP (PRMT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of A431 using 11279-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using PRMT1 antibody (11279-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 11279-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PRMT1 Polyclonal antibody (11279-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 11279-1-AP

Size/Concentration

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	A549 cells, mouse brain tissue, MCF-7 cells, HeLa cells, NIH/3T3 cells, rat brain tissue, Jurkat cells
Positive IP detected in	A549 cells
Positive IHC detected in	human colon tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 11 publications below
IHC	See 4 publications below
IF	See 1 publications below
IP	See 3 publications below

Product Information

11279-1-AP targets PRMT1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PRMT1 fusion protein Ag1790 相同性解析による交差性が予測される生物種
Full Name	protein arginine methyltransferase 1
Calculated molecular weight	42 kDa
Observed molecular weight	40-42 kDa
GenBank accession number	BC019268
Gene symbol	PRMT1
Gene ID (NCBI)	3276
RRID	AB_2171319
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PRMT1, the major protein arginine methyltransferase in mammals, catalyzes monomethylation and asymmetric dimethylation of arginine side chains in proteins. Human PRMT1 is encoded by the PRMT1 gene located on chromosome 19 (19q13.3) and composed of 12 exons and 11 introns. At the protein level, human PRMT1 shares a high degree of homology with the different members of the PRMT family that is conserved in eukaryotes. Phylogenetic studies based on the methyltransferase domain highlighted that PRMT1 is closely related to PRMT8.

Protocols

Product Specific Protocols
WB protocol for PRMT1 antibody 11279-1-AP	Download protocol
IHC protocol for PRMT1 antibody 11279-1-AP	Download protocol
IF protocol for PRMT1 antibody 11279-1-AP	Download protocol
IP protocol for PRMT1 antibody 11279-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human,mouse	WB,IHC,IP	Cell Metab Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners Authors - Qing Li View Article
mouse	WB,IP	Nucleic Acids Res RNA helicase D1PAS1 resolves R-loops and forms a complex for mouse pachytene piRNA biogenesis required for male fertility Authors - Heejin Choi View Article
human	WB	Cell Death Dis PRMT1-mediated PGK1 arginine methylation promotes colorectal cancer glycolysis and tumorigenesis Authors - Hao Liu View Article
human	IHC	Oncogene PRMT1 enhances oncogenic arginine methylation of NONO in colorectal cancer. Authors - Xin-Ke Yin View Article
human,mouse	WB,IHC	NPJ Precis Oncol The SMARCA4R1157W mutation facilitates chromatin remodeling and confers PRMT1/SMARCA4 inhibitors sensitivity in colorectal cancer Authors - Xiangwei Zeng View Article
mouse	WB	Diabetes PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARγ Authors - Yi Zhong View Article

Reviews

Add a review

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

Kyosuke (Verified Customer) (06-12-2019)

It works well on HUVECs for Western blot.

Applications: Western Blot,
Primary Antibody Dilution: 1/1000
Cell Tissue Type: HUVECs

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRMT1 Polyclonal antibody

PRMT1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Validation Data Gallery

WB analysis using 11279-1-AP

WB analysis of A549 using 11279-1-AP

WB analysis using 11279-1-AP

WB analysis using 11279-1-AP

IP experiment of A549 using 11279-1-AP

IHC staining of human colon using 11279-1-AP

IHC staining of human colon using 11279-1-AP

IF Staining of A431 using 11279-1-AP

FC experiment of HepG2 using 11279-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Thymidine kinase 1 drives hepatocellular carcinoma in enzyme-dependent and -independent manners

RNA helicase D1PAS1 resolves R-loops and forms a complex for mouse pachytene piRNA biogenesis required for male fertility

PRMT1-mediated PGK1 arginine methylation promotes colorectal cancer glycolysis and tumorigenesis

PRMT1 enhances oncogenic arginine methylation of NONO in colorectal cancer.

The SMARCA4R1157W mutation facilitates chromatin remodeling and confers PRMT1/SMARCA4 inhibitors sensitivity in colorectal cancer

PRMT4 Facilitates White Adipose Tissue Browning and Thermogenesis by Methylating PPARγ

Reviews