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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRMT1 Monoclonal antibody

PRMT1 Monoclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

1G8E2

Cat no : 68365-1-Ig

Synonyms

HRMT1L2, Histone-arginine N-methyltransferase PRMT1, HCP1, EC:2.1.1.319, ANM1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRMT1 Monoclonal antibody (68365-1-Ig)

    WB analysis using 68365-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68365-1-Ig (PRMT1 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.

  • Flow cytometry (FC) experiment of HeLa cells using PRMT1 Monoclonal antibody (68365-1-Ig)

    FC experiment of HeLa using 68365-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.2 ug PRMT1 Monoclonal antibody (68365-1-Ig, Clone:1G8E2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.2 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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Tested Applications

Positive WB detected inU2OS cells, LNCaP cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68365-1-Ig targets PRMT1 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PRMT1 fusion protein Ag29063 相同性解析による交差性が予測される生物種
Full Name protein arginine methyltransferase 1
Calculated molecular weight 42 kDa
Observed molecular weight38~42 kDa
GenBank accession numberBC019268
Gene symbol PRMT1
Gene ID (NCBI) 3276
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRMT1, the major protein arginine methyltransferase in mammals, catalyzes monomethylation and asymmetric dimethylation of arginine side chains in proteins. Human PRMT1 is encoded by the PRMT1 gene located on chromosome 19 (19q13.3) and composed of 12 exons and 11 introns. At the protein level, human PRMT1 shares a high degree of homology with the different members of the PRMT family that is conserved in eukaryotes. Phylogenetic studies based on the methyltransferase domain highlighted that PRMT1 is closely related to PRMT8.

Protocols

Product Specific Protocols
WB protocol for PRMT1 antibody 68365-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols