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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRMT3 Polyclonal antibody, PBS Only

PRMT3 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17628-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRMT3 Polyclonal antibody (17628-1-AP)

    WB analysis using 17628-1-AP (same clone as 17628-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17628-1-AP (PRMT3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17628-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PRMT3 Polyclonal antibody (17628-1-AP)

    IHC staining of mouse liver using 17628-1-AP (same clone as 17628-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17628-1-AP (PRMT3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17628-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PRMT3 Polyclonal antibody (17628-1-AP)

    IHC staining of mouse liver using 17628-1-AP (same clone as 17628-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 17628-1-AP (PRMT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17628-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRMT3 Polyclonal antibody (17628-1-AP)

    IF Staining of HeLa using 17628-1-AP (same clone as 17628-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRMT3 antibody (17628-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 17628-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17628-1-PBS targets PRMT3 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRMT3 fusion protein Ag11877 相同性解析による交差性が予測される生物種
Full Name protein arginine methyltransferase 3
Calculated molecular weight 531 aa, 60 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC037544
Gene symbol PRMT3
Gene ID (NCBI) 10196
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PRMT3(protein arginine methyltransferase 3) regulates protein functions by introducing asymmetric dimethylation marks at the arginine residues in proteins.