• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRMT3 Recombinant antibody, PBS Only (Detector)

PRMT3 Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241092F2

Cat no : 83969-3-PBS

Synonyms

Protein arginine N-methyltransferase 3, PRMT 3, HRMT1L3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, EC:2.1.1.319

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRMT3 Recombinant antibody (83969-3-RR)

    IF Staining of HeLa using 83969-3-RR (same clone as 83969-3-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PRMT3 antibody (83969-3-RR, Clone: 241092F2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83969-3-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A549 cells using PRMT3 Recombinant antibody (83969-3-RR)

    FC experiment of A549 using 83969-3-RR (same clone as 83969-3-PBS)

    1x10^6 A549 cells were intracellularly stained with 0.25 ug PRMT3 Recombinant antibody (83969-3-RR, Clone:241092F2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83969-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00892-1

    Cytometric bead array standard curve of MP00892-1

    Cytometric bead array standard curve of MP00892-1, PRMT3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83969-5-PBS. Detection antibody: 83969-3-PBS. Standard: Ag13466. Range: 0.781-100 ng/mL.

  • Cytometric bead array standard curve of MP00892-3

    Cytometric bead array standard curve of MP00892-3

    Cytometric bead array standard curve of MP00892-3, PRMT3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83969-2-PBS. Detection antibody: 83969-3-PBS. Standard: Ag13466. Range: 0.781-100 ng/mL.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83969-3-PBS targets PRMT3 as part of a matched antibody pair:

MP00892-1: 83969-5-PBS capture and 83969-3-PBS detection (validated in Cytometric bead array)

MP00892-3: 83969-2-PBS capture and 83969-3-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PRMT3 fusion protein Ag13466 相同性解析による交差性が予測される生物種
Full Name protein arginine methyltransferase 3
Calculated molecular weight 531 aa, 60 kDa
GenBank accession numberBC037544
Gene symbol PRMT3
Gene ID (NCBI) 10196
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PRMT3(protein arginine methyltransferase 3) regulates protein functions by introducing asymmetric dimethylation marks at the arginine residues in proteins.