• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRMT3 Recombinant antibody, PBS Only

PRMT3 Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

241092H2

Cat no : 83969-6-PBS

Synonyms

Protein arginine N-methyltransferase 3, PRMT 3, HRMT1L3, Heterogeneous nuclear ribonucleoprotein methyltransferase-like protein 3, EC:2.1.1.319

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PRMT3 Recombinant antibody (83969-6-RR)

    WB analysis using 83969-6-RR (same clone as 83969-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83969-6-RR (PRMT3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83969-6-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PRMT3 Recombinant antibody (83969-6-RR)

    IHC staining of mouse liver using 83969-6-RR (same clone as 83969-6-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 83969-6-RR (PRMT3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83969-6-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRMT3 Recombinant antibody (83969-6-RR)

    IF Staining of HepG2 using 83969-6-RR (same clone as 83969-6-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PRMT3 antibody (83969-6-RR, Clone: 241092H2 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83969-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using PRMT3 Recombinant antibody (83969-6-RR)

    FC experiment of MCF-7 using 83969-6-RR (same clone as 83969-6-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug PRMT3 Recombinant antibody (83969-6-RR, Clone:241092H2) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83969-6-PBS in a different storage buffer formulation.

☆対象製品が30%オフ！組換えタンパク質&免染用試薬 >>> キャンペーン実施中！

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83969-6-PBS is PRMT3 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PRMT3 fusion protein Ag13466 相同性解析による交差性が予測される生物種
Full Name protein arginine methyltransferase 3
Calculated molecular weight 531 aa, 60 kDa
Observed molecular weight60-68 kDa
GenBank accession numberBC037544
Gene symbol PRMT3
Gene ID (NCBI) 10196
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PRMT3(protein arginine methyltransferase 3) regulates protein functions by introducing asymmetric dimethylation marks at the arginine residues in proteins.