Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 18436-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.
WB analysis using 18436-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis using 18436-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 18436-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
WB analysis of Jurkat using 18436-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Raji using 18436-1-AP
Raji cells were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of mouse embryo using 18436-1-AP
mouse embryo tissue were subjected to SDS PAGE followed by western blot with 18436-1-AP (PRMT5 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of mouse brain using 18436-1-AP
IP result of anti-PRMT5 (IP:18436-1-AP, 4ug; Detection:18436-1-AP 1:2000) with mouse brain tissue lysate 1280 ug.
IHC staining of human breast cancer using 18436-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse liver using 18436-1-AP
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 18436-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 18436-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung using 18436-1-AP
Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 18436-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 18436-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 18436-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lymphoma using 18436-1-AP
Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 18436-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human lung cancer using 18436-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18436-1-AP (PRMT5 antibody) at dilution of 1:200 (under 40x lens).
IF Staining of HeLa using 18436-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PRMT5 antibody (18436-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of HepG2 using 18436-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PRMT5 antibody (18436-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HeLa using 18436-1-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human PRMT5 (18436-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

Featured Product
KD/KO Validated

PRMT5 Polyclonal antibody

PRMT5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 18436-1-AP

Publications(19)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

Protein arginine N-methyltransferase 5, Protein arginine N methyltransferase 5, Jak-binding protein 1, Histone-arginine N-methyltransferase PRMT5, EC:2.1.1.320

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (19)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HepG2 cells, HeLa cells, Jurkat cells, mouse embryo tissue, Raji cells, NIH/3T3 cells, mouse brain tissue, rat brain tissue
Positive IP detected in	mouse brain tissue
Positive IHC detected in	human breast cancer tissue, human lymphoma tissue, human lung tissue, human colon cancer tissue, human lung cancer tissue, mouse liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells, HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 7 publications below
WB	See 19 publications below
IHC	See 3 publications below
IF	See 2 publications below
IP	See 7 publications below
CoIP	See 1 publications below

Product Information

18436-1-AP targets PRMT5 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PRMT5 fusion protein Ag9736 相同性解析による交差性が予測される生物種
Full Name	protein arginine methyltransferase 5
Calculated molecular weight	637 aa, 73 kDa
Observed molecular weight	68-70 kDa
GenBank accession number	BC025979
Gene Symbol	PRMT5
Gene ID (NCBI)	10419
RRID	AB_2171798
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O14744
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PRMT5 is a Type II enzyme that catalyzes arginine monomethylation and symmetric dimethylation (Rme1 and Rme2s). The many targets of PRMT5 include ribosomal proteins, the histone chaperone nucleoplasmin, p53, and histones. PRMT5 is frequently observed in a complex with the cofactor, methylosome protein 50 (MEP50), which is required for PRMT5 activity. PRMT5 is upregulated in several human malignancies, including lymphomas, lung cancer, breast cancer and colorectal cancer.

Protocols

Product Specific Protocols
WB protocol for PRMT5 antibody 18436-1-AP	Download protocol
IHC protocol for PRMT5 antibody 18436-1-AP	Download protocol
IF protocol for PRMT5 antibody 18436-1-AP	Download protocol
IP protocol for PRMT5 antibody 18436-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB,IP,IF	Adv Sci (Weinh) TBL2 Promotes Tumorigenesis via PRMT5/WDR77-Mediated AKT Activation in Breast Cancer Authors - Xiuqing Lu View Article
human	WB	Sci Adv Arginine methyltransferase PRMT5 negatively regulates cGAS-mediated antiviral immune response. Authors - Dapeng Ma KD Validated View Article
human	WB	Nat Commun The LINC01138 drives malignancies via activating arginine methyltransferase 5 in hepatocellular carcinoma. Authors - Zhe Li View Article
human	WB,IHC	EMBO J MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression Authors - Yan Sun KD Validated View Article
human	WB,IP	Nat Commun USP9X regulates centrosome duplication and promotes breast carcinogenesis. Authors - Xin Li View Article
human	WB,IP	Cell Death Dis MYBL1 induces transcriptional activation of ANGPT2 to promote tumor angiogenesis and confer sorafenib resistance in human hepatocellular carcinoma Authors - Jinrong Zhu View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 18436-1-AP

WB analysis using 18436-1-AP

WB analysis using 18436-1-AP

WB analysis using 18436-1-AP

WB analysis of Jurkat using 18436-1-AP

WB analysis of Raji using 18436-1-AP

WB analysis of mouse embryo using 18436-1-AP

IP experiment of mouse brain using 18436-1-AP

IHC staining of human breast cancer using 18436-1-AP

IHC staining of mouse liver using 18436-1-AP

IHC staining of human breast cancer using 18436-1-AP

IHC staining of human lung using 18436-1-AP

IHC staining of human lung using 18436-1-AP

IHC staining of human colon cancer using 18436-1-AP

IHC staining of human colon cancer using 18436-1-AP

IHC staining of human lymphoma using 18436-1-AP

IHC staining of human lymphoma using 18436-1-AP

IHC staining of human lung cancer using 18436-1-AP

IHC staining of human lung cancer using 18436-1-AP

IF Staining of HeLa using 18436-1-AP

IF Staining of HepG2 using 18436-1-AP

FC experiment of HeLa using 18436-1-AP

PRMT5 Polyclonal antibody

PRMT5 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

TBL2 Promotes Tumorigenesis via PRMT5/WDR77-Mediated AKT Activation in Breast Cancer

Arginine methyltransferase PRMT5 negatively regulates cGAS-mediated antiviral immune response.

The LINC01138 drives malignancies via activating arginine methyltransferase 5 in hepatocellular carcinoma.

MST2 methylation by PRMT5 inhibits Hippo signaling and promotes pancreatic cancer progression

USP9X regulates centrosome duplication and promotes breast carcinogenesis.

MYBL1 induces transcriptional activation of ANGPT2 to promote tumor angiogenesis and confer sorafenib resistance in human hepatocellular carcinoma