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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRMT5 Polyclonal antibody

PRMT5 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 27109-1-AP

Synonyms

72 kDa ICln binding protein, HRMT1L5, IBP72, Jak binding protein 1, JBP1, PRMT5, SKB1, SKB1 homolog, SKB1Hs

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRMT5 Polyclonal antibody (27109-1-AP)

    WB analysis using 27109-1-AP

    Various cells were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PRMT5 Polyclonal antibody (27109-1-AP)

    WB analysis using 27109-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-12 cells using PRMT5 Polyclonal antibody (27109-1-AP)

    WB analysis of PC-12 using 27109-1-AP

    PC-12 cells were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using PRMT5 Polyclonal antibody (27109-1-AP)

    WB analysis of NIH/3T3 using 27109-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using PRMT5 Polyclonal antibody (27109-1-AP)

    WB analysis of Raji using 27109-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 27109-1-AP (PRMT5 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

Positive WB detected inHEK-293 cells, PC-12 cells, NIH/3T3 cells, Raji cells, HeLa cells, HepG2 cells, HL-60 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27109-1-AP targets PRMT5 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRMT5 fusion protein Ag25896 相同性解析による交差性が予測される生物種
Full Name protein arginine methyltransferase 5
Calculated molecular weight 637 aa, 73 kDa
Observed molecular weight 68 kDa
GenBank accession numberBC025979
Gene symbol PRMT5
Gene ID (NCBI) 10419
RRIDAB_2880760
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PRMT5 antibody 27109-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols