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  • Featured Product
  • KD/KO Validated

PRMT6 Polyclonal antibody, PBS Only

PRMT6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15395-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRMT6 Polyclonal antibody (15395-1-AP)

    WB analysis using 15395-1-AP (same clone as 15395-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15395-1-AP (PRMT6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using PRMT6 Polyclonal antibody (15395-1-AP)

    WB analysis of mouse brain using 15395-1-AP (same clone as 15395-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15395-1-AP (PRMT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PRMT6 Polyclonal antibody (15395-1-AP)

    IP experiment of HEK-293 using 15395-1-AP (same clone as 15395-1-PBS)

    IP result of anti-PRMT6 (IP:15395-1-AP, 4ug; Detection:15395-1-AP 1:1500) with HEK-293 cells lysate 2400ug. This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PRMT6 Polyclonal antibody (15395-1-AP)

    IHC staining of human prostate cancer using 15395-1-AP (same clone as 15395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PRMT6 Polyclonal antibody (15395-1-AP)

    IHC staining of human prostate cancer using 15395-1-AP (same clone as 15395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRMT6 Polyclonal antibody (15395-1-AP)

    IHC staining of human breast cancer using 15395-1-AP (same clone as 15395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRMT6 Polyclonal antibody (15395-1-AP)

    IHC staining of human breast cancer using 15395-1-AP (same clone as 15395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PRMT6 Polyclonal antibody (15395-1-AP)

    IHC staining of human kidney using 15395-1-AP (same clone as 15395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PRMT6 Polyclonal antibody (15395-1-AP)

    IHC staining of human kidney using 15395-1-AP (same clone as 15395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PRMT6 Polyclonal antibody (15395-1-AP)

    IHC staining of human testis using 15395-1-AP (same clone as 15395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PRMT6 Polyclonal antibody (15395-1-AP)

    IHC staining of human testis using 15395-1-AP (same clone as 15395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PRMT6 Polyclonal antibody (15395-1-AP)

    IF Staining of MCF-7 using 15395-1-AP (same clone as 15395-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PRMT6 antibody (15395-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using PRMT6 Polyclonal antibody (15395-1-AP)

    FC experiment of MCF-7 using 15395-1-AP (same clone as 15395-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug PRMT6 Polyclonal antibody (15395-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15395-1-PBS targets PRMT6 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRMT6 fusion protein Ag7592 相同性解析による交差性が予測される生物種
Full Name protein arginine methyltransferase 6
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC002729
Gene symbol PRMT6
Gene ID (NCBI) 55170
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PRMT6 belongs to the protein arginine methyltransferase (PRMT) family that catalyzes the mono- and dimethylation of arginine residues in a variety of proteins. PRMT6 is a nuclear protein and is predominantly expressed in testis and kidney. Western blot analysis using this antibody detected a major band around 42 kDa in HeLa cells.