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  • Featured Product
  • KD/KO Validated

PRMT6 Polyclonal antibody, PBS Only

PRMT6 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 15395-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRMT6 Polyclonal antibody (15395-1-AP)

    WB analysis using 15395-1-AP (same clone as 15395-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 15395-1-AP (PRMT6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using PRMT6 Polyclonal antibody (15395-1-AP)

    WB analysis of mouse brain using 15395-1-AP (same clone as 15395-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 15395-1-AP (PRMT6 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using PRMT6 Polyclonal antibody (15395-1-AP)

    IP experiment of HEK-293 using 15395-1-AP (same clone as 15395-1-PBS)

    IP result of anti-PRMT6 (IP:15395-1-AP, 4ug; Detection:15395-1-AP 1:1500) with HEK-293 cells lysate 2400ug. This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PRMT6 Polyclonal antibody (15395-1-AP)

    IHC staining of human prostate cancer using 15395-1-AP (same clone as 15395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PRMT6 Polyclonal antibody (15395-1-AP)

    IHC staining of human prostate cancer using 15395-1-AP (same clone as 15395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PRMT6 Polyclonal antibody (15395-1-AP)

    IHC staining of human testis using 15395-1-AP (same clone as 15395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using PRMT6 Polyclonal antibody (15395-1-AP)

    IHC staining of human testis using 15395-1-AP (same clone as 15395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRMT6 Polyclonal antibody (15395-1-AP)

    IHC staining of human breast cancer using 15395-1-AP (same clone as 15395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRMT6 Polyclonal antibody (15395-1-AP)

    IHC staining of human breast cancer using 15395-1-AP (same clone as 15395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15395-1-AP (PRMT6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PRMT6 Polyclonal antibody (15395-1-AP)

    IHC staining of human kidney using 15395-1-AP (same clone as 15395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PRMT6 Polyclonal antibody (15395-1-AP)

    IHC staining of human kidney using 15395-1-AP (same clone as 15395-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human kidney using 15395-1-AP (PRMT6 antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PRMT6 Polyclonal antibody (15395-1-AP)

    IF Staining of MCF-7 using 15395-1-AP (same clone as 15395-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PRMT6 antibody (15395-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using PRMT6 Polyclonal antibody (15395-1-AP)

    FC experiment of MCF-7 using 15395-1-AP (same clone as 15395-1-PBS)

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug PRMT6 Polyclonal antibody (15395-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 15395-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

15395-1-PBS targets PRMT6 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRMT6 fusion protein Ag7592 相同性解析による交差性が予測される生物種
Full Name protein arginine methyltransferase 6
Calculated molecular weight 42 kDa
Observed molecular weight 42 kDa
GenBank accession numberBC002729
Gene symbol PRMT6
Gene ID (NCBI) 55170
RRIDAB_2237723
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PRMT6 belongs to the protein arginine methyltransferase (PRMT) family that catalyzes the mono- and dimethylation of arginine residues in a variety of proteins. PRMT6 is a nuclear protein and is predominantly expressed in testis and kidney. Western blot analysis using this antibody detected a major band around 42 kDa in HeLa cells.