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Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using PRMT8 Polyclonal antibody (12557-1-AP)

    WB analysis of Y79 using 12557-1-AP (same clone as 12557-1-PBS)

    Y79 cells were subjected to SDS PAGE followed by western blot with 12557-1-AP (PRMT8 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12557-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using PRMT8 Polyclonal antibody (12557-1-AP)

    IHC staining of rat brain using 12557-1-AP (same clone as 12557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 12557-1-AP (PRMT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12557-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PRMT8 Polyclonal antibody (12557-1-AP)

    IHC staining of mouse brain using 12557-1-AP (same clone as 12557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12557-1-AP (PRMT8 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12557-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PRMT8 Polyclonal antibody (12557-1-AP)

    IHC staining of mouse brain using 12557-1-AP (same clone as 12557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12557-1-AP (PRMT8 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12557-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using PRMT8 Polyclonal antibody (12557-1-AP)

    IHC staining of human breast cancer using 12557-1-AP (same clone as 12557-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12557-1-AP (PRMT8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12557-1-PBS in a different storage buffer formulation.

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PRMT8 Polyclonal antibody, PBS Only

PRMT8 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 12557-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12557-1-PBS targets PRMT8 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRMT8 fusion protein Ag3166 相同性解析による交差性が予測される生物種
Full Name protein arginine methyltransferase 8
Calculated molecular weight 394 aa, 45 kDa
Observed molecular weight 43-50 kDa
GenBank accession numberBC022458
Gene Symbol PRMT8
Gene ID (NCBI) 56341
RRIDAB_2171964
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NR22
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PRMT8 is a member of arginine methyl transferase (PRMT) family which acts as nuclear receptor coactivators. PRMT8 catalyzes the asymmetric arginine methylation and is involved in cellular differentiation. PRMT8 has several isoforms; the full-length isoform has a brain specific expression pattern and is localized to the plasma membrane; the truncated isoforms display nuclear localization and are expressed more widely. This antibody recognizes all of isoforms of PRMT8.