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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRND Polyclonal antibody

PRND Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, Rat and More (1)

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 26947-1-AP

Synonyms

DOPPEL, DPL, Prion like protein doppel, Prion protein 2, prion protein 2 (dublet), PRND, PrPLP

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRND Polyclonal antibody (26947-1-AP)

    WB analysis using 26947-1-AP

    Non-treated mouse testis and glucosidase treated mouse testis tissue were subjected to SDS PAGE followed by western blot with 26947-1-AP (PRND antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PRND Polyclonal antibody (26947-1-AP)

    WB analysis using 26947-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 26947-1-AP (PRND antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PRND Polyclonal antibody (26947-1-AP)

    IHC staining of mouse testis using 26947-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26947-1-AP (PRND antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PRND Polyclonal antibody (26947-1-AP)

    IHC staining of mouse testis using 26947-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 26947-1-AP (PRND antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using PRND Polyclonal antibody (26947-1-AP)

    IF Staining of mouse testis using 26947-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PRND antibody (26947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using PRND Polyclonal antibody (26947-1-AP)

    IF Staining of mouse testis using 26947-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse testis tissue using PRND antibody (26947-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inGlucosidase treated mouse testis tissue, mouse testis tissue, rat testis tissue
Positive IHC detected inmouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

The immunogen of 26947-1-AP is PRND Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, Rat
Cited Reactivity pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRND fusion protein Ag25523 相同性解析による交差性が予測される生物種
Full Name prion protein 2 (dublet)
Calculated molecular weight 176 aa, 20 kDa
Observed molecular weight17-20, 30 kDa
GenBank accession numberBC043644
Gene symbol PRND
Gene ID (NCBI) 23627
RRIDAB_2880695
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The prion gene family is mainly composed of three genes; PRNP, encoding the prion protein (PrP), PRND, encoding Doppel (Dpl), both located on the same genomic locus on mouse chromosome 2 and SPRN, encoding Shadoo (Sho), located on mouse chromosome 7. PRND (prion protein 2 also called Doppel) is a membranean N-glycosylated, glycosylphosphatidylinositol-anchored protein that is found predominantly in testis. PRND gene is found on chromosome 20, approximately 20 kbp downstream of the gene encoding cellular prionprotein, to which it is biochemically and structurally similar. 26947-1-AP antibody detects a 30 kDa glycosylated protein and a 17-20 kDa protein treated by glucosidase.

Protocols

Product Specific Protocols
WB protocol for PRND antibody 26947-1-APDownload protocol
IHC protocol for PRND antibody 26947-1-APDownload protocol
IF protocol for PRND antibody 26947-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
pigIF

J Genet Genomics

Single-cell RNA-seq analysis of testicular somatic cell development in pigs.

Authors - Lingkai Zhang
View Article