• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PROC Polyclonal antibody

PROC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 25382-1-AP

Synonyms

Vitamin K-dependent protein C, Vitamin K dependent protein C, EC:3.4.21.69, Blood coagulation factor XIV, Autoprothrombin IIA

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse liver tissue using PROC Polyclonal antibody (25382-1-AP)

    WB analysis of mouse liver using 25382-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25382-1-AP (PROC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using PROC Polyclonal antibody (25382-1-AP)

    IHC staining of human liver using 25382-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25382-1-AP (PROC Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PROC Polyclonal antibody (25382-1-AP)

    IHC staining of human liver using 25382-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25382-1-AP (PROC Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PROC Polyclonal antibody (25382-1-AP)

    IF Staining of HeLa using 25382-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PROC antibody (25382-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

☆ 2/28（金）まで！最大30%オフ！ >> トライアルサイズもお得な一次抗体キャンペーン

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

25382-1-AP targets PROC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PROC fusion protein Ag11328 相同性解析による交差性が予測される生物種
Full Name protein C (inactivator of coagulation factors Va and VIIIa)
Calculated molecular weight 461 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC034377
Gene symbol PROC
Gene ID (NCBI) 5624
RRIDAB_2880052
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PROC belongs to the peptidase S1 family. PROC is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PMID:25618265). It exerts a protective effect on the endothelial cell barrier function (PMID:25651845).

Protocols

Product Specific Protocols
WB protocol for PROC antibody 25382-1-APDownload protocol
IHC protocol for PROC antibody 25382-1-APDownload protocol
IF protocol for PROC antibody 25382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Cell Dev Biol

The predictive effect of immune therapy and chemotherapy under T cell-related gene prognostic index for Gastric cancer

Authors - Jingyao Chen
View Article