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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse liver tissue using PROC Polyclonal antibody (25382-1-AP)

    WB analysis of mouse liver using 25382-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 25382-1-AP (PROC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using PROC Polyclonal antibody (25382-1-AP)

    IHC staining of human liver using 25382-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25382-1-AP (PROC Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using PROC Polyclonal antibody (25382-1-AP)

    IHC staining of human liver using 25382-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 25382-1-AP (PROC Antibody) at dilution of 1:200 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PROC Polyclonal antibody (25382-1-AP)

    IF Staining of HeLa using 25382-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PROC antibody (25382-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

PROC Polyclonal antibody

PROC Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 25382-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Vitamin K-dependent protein C, Vitamin K dependent protein C, EC:3.4.21.69, Blood coagulation factor XIV, Autoprothrombin IIA

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse liver tissue
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

25382-1-AP targets PROC in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PROC fusion protein Ag11328 相同性解析による交差性が予測される生物種
Full Name protein C (inactivator of coagulation factors Va and VIIIa)
Calculated molecular weight 461 aa, 52 kDa
Observed molecular weight 52 kDa
GenBank accession numberBC034377
Gene Symbol PROC
Gene ID (NCBI) 5624
RRIDAB_2880052
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP04070
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PROC belongs to the peptidase S1 family. PROC is a vitamin K-dependent serine protease that regulates blood coagulation by inactivating factors Va and VIIIa in the presence of calcium ions and phospholipids (PMID:25618265). It exerts a protective effect on the endothelial cell barrier function (PMID:25651845).

Protocols

Product Specific Protocols
WB protocol for PROC antibody 25382-1-APDownload protocol
IHC protocol for PROC antibody 25382-1-APDownload protocol
IF protocol for PROC antibody 25382-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Front Cell Dev Biol

The predictive effect of immune therapy and chemotherapy under T cell-related gene prognostic index for Gastric cancer

Authors - Jingyao Chen
View Article