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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PROCR Monoclonal antibody (67658-1-Ig)

    WB analysis using 67658-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67658-1-Ig (PROCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PROCR Monoclonal antibody (67658-1-Ig)

    WB analysis using 67658-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67658-1-Ig (PROCR antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PROCR Monoclonal antibody (67658-1-Ig)

    IHC staining of human tonsillitis using 67658-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PROCR Monoclonal antibody (67658-1-Ig)

    IHC staining of human tonsillitis using 67658-1-Ig

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 67658-1-Ig (PROCR antibody) at dilution of 1:6000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of A549 cells using EPCR/CD201 Monoclonal antibody (67658-1-Ig)

    FC experiment of A549 using 67658-1-Ig

    1x10^6 A549 cells were intracellularly stained with 0.4 ug PROCR Monoclonal antibody (67658-1-Ig, Clone:1F5C4) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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EPCR/CD201 Monoclonal antibody

EPCR/CD201 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Cat No. 67658-1-Ig
Clone No.1F5C4
Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

human, pig and More (1)

Applications

WB, IHC, IF, FC (Intra), ELISA

Synonyms

CD201, EPCR, PROCR, 1F5C4, Activated protein C receptor

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman placenta tissue, pig spleen tissue, pig heart tissue, pig vein tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:3000-1:12000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67658-1-Ig targets EPCR/CD201 in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, pig samples.

Tested Reactivity human, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EPCR/CD201 fusion protein Ag25628 相同性解析による交差性が予測される生物種
Full Name protein C receptor, endothelial (EPCR)
Calculated molecular weight 238 aa, 27 kDa
Observed molecular weight49 kDa
GenBank accession numberBC014451
Gene Symbol PROCR
Gene ID (NCBI) 10544
RRIDAB_2882855
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9UNN8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for EPCR/CD201 antibody 67658-1-IgDownload protocol
IHC protocol for EPCR/CD201 antibody 67658-1-IgDownload protocol
FC protocol for EPCR/CD201 antibody 67658-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IHC,IF

Signal Transduct Target Ther

Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma.

Authors - Panpan Zhang
View Article