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KD/KO Validated

PRODH Polyclonal antibody

Name: PRODH Polyclonal antibody
Brand: Proteintech
SKU: 22980-1-AP

PRODH Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 22980-1-AP

Synonyms

HSPOX2, p53 induced gene 6 protein, PIG6, POX, POX2, PRODH, PRODH1, Proline oxidase, Proline oxidase 2, SCZD4, TP53I6

Validation Data Gallery

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AllWBIHC

WB analysis using 22980-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22980-1-AP (PRODH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 22980-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22980-1-AP (PRODH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 22980-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 22980-1-AP (PRODH antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
IHC staining of human cerebellum using 22980-1-AP
Immunohistochemical analysis of paraffin-embedded human cerebellum slide using 22980-1-AP (PRODH Antibody) at dilution of 1:50(under 10x lens).
IHC staining of human cerebellum using 22980-1-AP
Immunohistochemical analysis of paraffin-embedded human cerebellum slide using 22980-1-AP (PRODH Antibody) at dilution of 1:50(under 40x lens).
IHC staining of human liver cancer using 22980-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22980-1-AP (PRODH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 22980-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22980-1-AP (PRODH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 22980-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22980-1-AP (PRODH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 22980-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 22980-1-AP (PRODH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 22980-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22980-1-AP (PRODH antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 22980-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22980-1-AP (PRODH antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human skeletal muscle using 22980-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22980-1-AP (PRODH Antibody) at dilution of 1:50(under 10x lens).
IHC staining of human skeletal muscle using 22980-1-AP
Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 22980-1-AP (PRODH Antibody) at dilution of 1:50(under 40x lens).

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Cat No. 22980-1-AP

Size/Concentration

ご購入について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	mouse liver tissue, A549 cells, rat liver tissue
Positive IHC detected in	human cerebellum tissue, human liver cancer tissue, human breast cancer tissue, human skeletal muscle tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 14 publications below
IHC	See 3 publications below
IF	See 4 publications below

Product Information

22980-1-AP targets PRODH in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PRODH fusion protein Ag18429 相同性解析による交差性が予測される生物種
Full Name	proline dehydrogenase (oxidase) 1
Calculated molecular weight	600 aa, 68 kDa
Observed molecular weight	56 kDa, 66 kDa
GenBank accession number	BC118597
Gene symbol	PRODH
Gene ID (NCBI)	5625
RRID	AB_2879191
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PRODH(Proline dehydrogenase 1, mitochondrial) is also named as PIG6, HSPOX2, PRODH1, PRODH2, POX, SCZD4, TP53I6 and belongs to the proline oxidase family. It is an oxidoreductase involved in the transfer of redox potential across the mitochondrial membrane and catalyzes the rate-limiting oxidation of proline to pyrroline- 5-carboxylate (P5C). High PRODH activity is sufficient to induce mitochondria-mediated apoptosis in the presence of proline. Defects in PRODH are the cause of hyperprolinemia type 1 (HP-1) and defects in PRODH are associated with susceptibility to schizophrenia type 4 (SCZD4). This protein has 3 isoforms produced by alternative splicing with the molecular mass of 68 kDa, 59 kDa and 56 kDa.

Protocols

Product Specific Protocols
WB protocol for PRODH antibody 22980-1-AP	Download protocol
IHC protocol for PRODH antibody 22980-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nucleic Acids Res Discordant regulation of eIF2 kinase GCN2 and mTORC1 during nutrient stress. Authors - Jagannath Misra View Article
human	WB	Cell Rep Proline restores mitochondrial function and reverses aging hallmarks in senescent cells Authors - Debanik Choudhury View Article
rat	WB	Oxid Med Cell Longev Metabolomic Analysis of the Ameliorative Effect of Enhanced Proline Metabolism on Hypoxia-Induced Injury in Cardiomyocytes. Authors - Jiacheng Wang KD Validated View Article
human	WB	Amino Acids PINCH-1 promotes Δ1-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma. Authors - Chunhong Cui View Article
human	WB	Amino Acids Activation of proline metabolism maintains ATP levels during cocaine-induced polyADP-ribosylation. Authors - Sabyasachi Dash KD Validated View Article
	WB,IF	Animals (Basel) Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine. Authors - Chengwen Feng View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRODH Polyclonal antibody

PRODH Polyclonal Antibody for WB, IHC, ELISA

Validation Data Gallery

WB analysis using 22980-1-AP

WB analysis using 22980-1-AP

WB analysis using 22980-1-AP

IHC staining of human cerebellum using 22980-1-AP

IHC staining of human cerebellum using 22980-1-AP

IHC staining of human liver cancer using 22980-1-AP

IHC staining of human liver cancer using 22980-1-AP

IHC staining of human liver cancer using 22980-1-AP

IHC staining of human liver cancer using 22980-1-AP

IHC staining of human breast cancer using 22980-1-AP

IHC staining of human breast cancer using 22980-1-AP

IHC staining of human skeletal muscle using 22980-1-AP

IHC staining of human skeletal muscle using 22980-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Discordant regulation of eIF2 kinase GCN2 and mTORC1 during nutrient stress.

Proline restores mitochondrial function and reverses aging hallmarks in senescent cells

Metabolomic Analysis of the Ameliorative Effect of Enhanced Proline Metabolism on Hypoxia-Induced Injury in Cardiomyocytes.

PINCH-1 promotes Δ1-pyrroline-5-carboxylate synthase expression and contributes to proline metabolic reprogramming in lung adenocarcinoma.

Activation of proline metabolism maintains ATP levels during cocaine-induced polyADP-ribosylation.

Proline Protects Boar Sperm against Oxidative Stress through Proline Dehydrogenase-Mediated Metabolism and the Amine Structure of Pyrrolidine.