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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRODH2 Polyclonal antibody (31047-1-AP)

    WB analysis using 31047-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31047-1-AP (PRODH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PRODH2 Polyclonal antibody (31047-1-AP)

    WB analysis using 31047-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31047-1-AP (PRODH2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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PRODH2 Polyclonal antibody

PRODH2 Polyclonal Antibody for WB, ELISA
Cat No. 31047-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, ELISA

Synonyms

HSPOX1, Probable proline oxidase 2, PRODH2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse kidney tissue, human testis tissue, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

31047-1-AP targets PRODH2 in WB, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRODH2 fusion protein Ag33970 相同性解析による交差性が予測される生物種
Full Name proline dehydrogenase (oxidase) 2
Observed molecular weight 50 kDa
GenBank accession numberBC166629
Gene Symbol PRODH2
Gene ID (NCBI) 58510
RRIDAB_3669831
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UF12
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRODH2 is a dehydrogenase that converts trans-4-L-hydroxyproline to delta-1-pyrroline-3-hydroxy-5-carboxylate (Hyp) using ubiquinone-10 as the terminal electron acceptor. It can also use proline as a substrate but with a very much lower efficiency (PMID: 25697095).

Protocols

Product Specific Protocols
WB protocol for PRODH2 antibody 31047-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols