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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CD133-1,2,4,7 Polyclonal antibody

CD133-1,2,4,7 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19945-1-AP

Synonyms

AC133, Antigen AC133, CD133, CD133-1,2,4,7, CORD12, MCDR2, MSTP061, PROM1, PROM1 1, PROM1 1,2,3, PROM1 1,6,7, PROM1 2, PROM1 2,3,4,5, prominin 1, Prominin like protein 1, PROML1, RP41, STGD4

Validation Data Gallery

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  • Western Blot (WB) analysis of Y79 cells using CD133-1,2,4,7 Polyclonal antibody (19945-1-AP)

    WB analysis of Y79 using 19945-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 19945-1-AP (PROM1-1,2,3,5,7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse lung tissue using CD133-1,2,4,7 Polyclonal antibody (19945-1-AP)

    WB analysis of mouse lung using 19945-1-AP

    mouse lung tissue were subjected to SDS PAGE followed by western blot with 19945-1-AP (PROM1-1,2,3,5,7 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of Y79 cells using CD133-1,2,4,7 Polyclonal antibody (19945-1-AP)

    IP experiment of Y79 using 19945-1-AP

    IP result of anti-CD133-1,2,4,7 (IP:19945-1-AP, 4ug; Detection:19945-1-AP 1:300) with Y79 cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CD133-1,2,4,7 Polyclonal antibody (19945-1-AP)

    IHC staining of human gliomas using 19945-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19945-1-AP (CD133-1,2,4,7 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using CD133-1,2,4,7 Polyclonal antibody (19945-1-AP)

    IHC staining of human gliomas using 19945-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 19945-1-AP (CD133-1,2,4,7 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inY79 cells, mouse lung tissue
Positive IP detected inY79 cells
Positive IHC detected inhuman gliomas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19945-1-AP targets CD133-1,2,4,7 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name prominin 1
Calculated molecular weight 97 kDa
Observed molecular weight 115 kDa
GenBank accession numberNM_001145851
Gene symbol CD133
Gene ID (NCBI) 8842
RRIDAB_10644154
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD133, also known as PROM1 (prominin-1) or AC133, belongs to the prominin family. CD133 is a transmembrane glycoprotein with an NH2-terminal extracellular domain, five transmembrane loops and a cytoplasmic tail. The expression of CD133 has been reported in hematopoietic stem cells, endothelial progenitor cells, neuronal and glial stem cells, suggesting the potential role of CD133 as a cell surface marker of adult stem cells. CD133 has also been reported as a marker of cancer stem cells in various human tumors. This antibody recognizes transcript variant 1,2,4,7 (UNIPROT has updated the info of isoforms)of CD133.

Protocols

Product Specific Protocols
WB protocol for CD133-1,2,4,7 antibody 19945-1-APDownload protocol
IHC protocol for CD133-1,2,4,7 antibody 19945-1-APDownload protocol
IP protocol for CD133-1,2,4,7 antibody 19945-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols