Validation Data Gallery
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Tested Applications
| Positive WB detected in | HT-29 cells, MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20727-1-AP targets PROM2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | prominin 2 |
| Calculated molecular weight | 92 kDa |
| Observed molecular weight | 40 kDa |
| GenBank accession number | NM_144707 |
| Gene Symbol | PROM2 |
| Gene ID (NCBI) | 150696 |
| RRID | AB_3085619 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N271 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PROM2, also named as prominin 2, encodes a member of the prominin family of pentaspan membrane glycoproteins. The encoded protein localizes to basal epithelial cells and may be involved in the organization of plasma membrane microdomains. Alternative splicing results in multiple transcript variants. PROM2 has 2 isoforms with the MW of 86 kDa and 41 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PROM2 antibody 20727-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |