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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PROX1 Recombinant antibody

PROX1 Recombinant Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2H12

Cat no : 81778-1-RR

Synonyms

PROX-1, Prospero homeobox protein 1, prospero homeobox 1, Homeobox prospero-like protein PROX1, 2H12

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PROX1 Recombinant antibody (81778-1-RR)

    WB analysis using 81778-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81778-1-RR (PROX1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HuH-7 cells using PROX1 Recombinant antibody (81778-1-RR)

    IF Staining of HuH-7 using 81778-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HuH-7 cells using PROX1 antibody (81778-1-RR, Clone: 2H12 ) at dilution of 1:1300 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), PROX1 antibody (67438-1-Ig, Clone: 1D4B1, red).

  • Affinity and Kinetic Characterization of 81778-1-RR

    Affinity and Kinetic Characterization of 81778-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81778-1-RR against Human PROX1 were performed. The affinity constant is 26.4 nM.

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Tested Applications

Positive WB detected inHepG2 cells, HuH-7 cells, SH-SY5Y cells
Positive IF/ICC detected inHuH-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:650-1:2600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

81778-1-RR targets PROX1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PROX1 fusion protein Ag1543 相同性解析による交差性が予測される生物種
Full Name prospero homeobox 1
Calculated molecular weight 737 aa, 83 kDa
Observed molecular weight 90 kDa
GenBank accession numberBC024201
Gene symbol PROX1
Gene ID (NCBI) 5629
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PROX1, Prospero homeobox protein 1, belongs to the Prospero homeobox family and contain a Prospero-type homeobox DNA-binding domain. PROX1 plays a fundamental role in early development of CNS and regulates the expression of gene and development of postmitotic, undifferentiated neurons. Also, PROX1 is a required regulator of the development of the lymphatic system. This antibody is raised against part of C-terminal chain of human PROX1. The calculated molecular weight of PROX1 is 83 kDa, but with post-translational modifications, PROX1 is about 90 kDa.

Protocols

Product Specific Protocols
WB protocol for PROX1 antibody 81778-1-RRDownload protocol
IF protocol for PROX1 antibody 81778-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols