• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRPF19 Polyclonal antibody

PRPF19 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15414-1-AP

Synonyms

RING-type E3 ubiquitin transferase PRP19, PSO4, PRP19, Pre-mRNA-processing factor 19, hPso4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of mouse kidney tissue using PRPF19 Polyclonal antibody (15414-1-AP)

    WB analysis of mouse kidney using 15414-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of rat kidney tissue using PRPF19 Polyclonal antibody (15414-1-AP)

    WB analysis of rat kidney using 15414-1-AP

    rat kidney tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PRPF19 Polyclonal antibody (15414-1-AP)

    WB analysis of HEK-293 using 15414-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PRPF19 Polyclonal antibody (15414-1-AP)

    WB analysis of HeLa using 15414-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using PRPF19 Polyclonal antibody (15414-1-AP)

    WB analysis of human testis using 15414-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using PRPF19 Polyclonal antibody (15414-1-AP)

    WB analysis of PC-3 using 15414-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using PRPF19 Polyclonal antibody (15414-1-AP)

    WB analysis of mouse liver using 15414-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 15414-1-AP (PRPF19 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach tissue using PRPF19 Polyclonal antibody (15414-1-AP)

    IHC staining of human stomach using 15414-1-AP

    Immunohistochemical analysis of paraffin-embedded human normal stomach slide using 15414-1-AP (PRPF19 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

☆大好評トライアルサイズ ¥20,000～！ >>> 一次抗体特別価格キャンペーン開催中！

Tested Applications

Positive WB detected inmouse kidney tissue, HEK-293 cells, HeLa cells, human testis tissue, mouse liver tissue, PC-3 cells, rat kidney tissue
Positive IHC detected inhuman stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 3 publications below

Product Information

15414-1-AP targets PRPF19 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRPF19 fusion protein Ag7700 相同性解析による交差性が予測される生物種
Full Name PRP19/PSO4 pre-mRNA processing factor 19 homolog (S. cerevisiae)
Calculated molecular weight 55 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC008719
Gene symbol PRPF19
Gene ID (NCBI) 27339
RRIDAB_10637855
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRPF19 is involved in several processes, including DNA damage checkpoint signaling; cellular protein metabolic process; and mRNA splicing, via spliceosome.

Protocols

Product Specific Protocols
WB protocol for PRPF19 antibody 15414-1-APDownload protocol
IHC protocol for PRPF19 antibody 15414-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Expert Opin Ther Targets

Proteome of thymus and spleen reveals that 10-hydroxydec-2-enoic acid could enhance immunity in mice.

Authors - Pei Fan
View Article
humanWB

J Cell Biochem

Human RNF113A participates of pre-mRNA splicing in vitro.

Authors - Guilherme H Gatti da Silva
View Article
human,pigWB

J Virol

PRPF19 Limits Porcine Epidemic Diarrhea Virus Replication through Targeting and Degrading Viral Capsid Protein

Authors - Xueying Zhai
View Article