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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRPF31 Polyclonal antibody

PRPF31 Polyclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

Cat no : 27750-1-AP

Synonyms

RP11, PRP31, Protein 61K, NY BR 99, hPrp31

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PRPF31 Polyclonal antibody (27750-1-AP)

    WB analysis using 27750-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27750-1-AP (PRPF31 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PRPF31 Polyclonal antibody (27750-1-AP)

    IHC staining of mouse eye using 27750-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 27750-1-AP (PRPF31 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PRPF31 Polyclonal antibody (27750-1-AP)

    IHC staining of mouse eye using 27750-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 27750-1-AP (PRPF31 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PRPF31 Polyclonal antibody (27750-1-AP)

    IF Staining of mouse eye using 27750-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using PRPF31 antibody (27750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PRPF31 Polyclonal antibody (27750-1-AP)

    IF Staining of mouse eye using 27750-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using PRPF31 antibody (27750-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inA549 cells, Caco-2 cells, Hela cells, Jurkat cells
Positive IHC detected inmouse eye tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse eye tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27750-1-AP targets PRPF31 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRPF31 fusion protein Ag26716 相同性解析による交差性が予測される生物種
Full Name PRP31 pre-mRNA processing factor 31 homolog (S. cerevisiae)
Calculated molecular weight 55 kDa
Observed molecular weight55 kDa
GenBank accession numberNM_015629
Gene symbol PRPF31
Gene ID (NCBI) 26121
RRIDAB_2918130
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRPF31 is a component of the spliceosome complex. It is recruited to introns following the attachment of U4 and U6 RNAs and the 15.5K protein. The addition of PRPF31 is crucial for the transition of the spliceosomal complex to the activated state.

Protocols

Product Specific Protocols
WB protocol for PRPF31 antibody 27750-1-APDownload protocol
IHC protocol for PRPF31 antibody 27750-1-APDownload protocol
IF protocol for PRPF31 antibody 27750-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols