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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRPF38A Polyclonal antibody

PRPF38A Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 24946-1-AP

Synonyms

RP5 965L7.1, Pre-mRNA-splicing factor 38A, Pre mRNA splicing factor 38A

Validation Data Gallery

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  • Western Blot (WB) analysis of K-562 cells using PRPF38A Polyclonal antibody (24946-1-AP)

    WB analysis of K-562 using 24946-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 24946-1-AP (PRPF38A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PRPF38A Polyclonal antibody (24946-1-AP)

    WB analysis of HepG2 using 24946-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 24946-1-AP (PRPF38A Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRPF38A Polyclonal antibody (24946-1-AP)

    IF Staining of HepG2 using 24946-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 24946-1-AP (PRPF38A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inK-562 cells, HepG2 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

24946-1-AP targets PRPF38A in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRPF38A fusion protein Ag20980 相同性解析による交差性が予測される生物種
Full Name PRP38 pre-mRNA processing factor 38 (yeast) domain containing A
Calculated molecular weight 312 aa, 37 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC000777
Gene symbol PRPF38A
Gene ID (NCBI) 84950
RRIDAB_2879814
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRPF38A was identified as an essential splicing factor in yeast and was found to be important for the progression of the splicing process to the first splicing step in this organism. PRPF38A is one of nine splicing factors that are specifically incorporated during the formation of the pre-catalytic spliceosomal B complex (B-specific proteins) and that are released again during spliceosome activation3 (PMID: 35869234). PRPF38A knockdown led to widespread intronic retention and altered splicing of transcripts of genes implicated in protein metabolism, mitosis, proteasome function and apoptosis (PMID: 28878028).

Protocols

Product Specific Protocols
WB protocol for PRPF38A antibody 24946-1-APDownload protocol
IF protocol for PRPF38A antibody 24946-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols