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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRPF39 Polyclonal antibody

PRPF39 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 24480-1-AP

Synonyms

Pre mRNA processing factor 39, PRP39 homolog, PRPF39

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of K-562 cells using PRPF39 Polyclonal antibody (24480-1-AP)

    WB analysis of K-562 using 24480-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 24480-1-AP (PRPF39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using PRPF39 Polyclonal antibody (24480-1-AP)

    WB analysis of Raji using 24480-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 24480-1-AP (PRPF39 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using PRPF39 Polyclonal antibody (24480-1-AP)

    WB analysis of COLO 320 using 24480-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 24480-1-AP (PRPF39 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using PRPF39 Polyclonal antibody (24480-1-AP)

    IHC staining of human testis using 24480-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:400 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PRPF39 Polyclonal antibody (24480-1-AP)

    IHC staining of human testis using 24480-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PRPF39 Polyclonal antibody (24480-1-AP)

    IHC staining of human testis using 24480-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PRPF39 Polyclonal antibody (24480-1-AP)

    IHC staining of human testis using 24480-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PRPF39 Polyclonal antibody (24480-1-AP)

    IHC staining of human testis using 24480-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PRPF39 Polyclonal antibody (24480-1-AP)

    IHC staining of human testis using 24480-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 24480-1-AP (PRPF39 Antibody) at dilution of 1:400 (under 10x lens).

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Tested Applications

Positive WB detected inK-562 cells, COLO 320 cells, Raji cells
Positive IHC detected inhuman testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 24480-1-AP is PRPF39 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRPF39 fusion protein Ag21683 相同性解析による交差性が予測される生物種
Full Name PRP39 pre-mRNA processing factor 39 homolog (S. cerevisiae)
Calculated molecular weight 669 aa, 78 kDa
Observed molecular weight 75-85 kDa
GenBank accession numberBC125126
Gene symbol PRPF39
Gene ID (NCBI) 55015
RRIDAB_2879563
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRPF39 also termed as Pre mRNA processing factor 39 or PRP39 homolog is a 669 amino acid protein, which contains 7 HAT repeats and belongs to the PRP39 family. PRPF39 localizes in nucleus and may play a role in mRNA splicing.

Protocols

Product Specific Protocols
WB protocol for PRPF39 antibody 24480-1-APDownload protocol
IHC protocol for PRPF39 antibody 24480-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols