PRPF8 Polyclonal antibody

Name: PRPF8 Polyclonal antibody
Brand: Proteintech
SKU: 11171-1-AP

PRPF8 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

Review Product

Cat no : 11171-1-AP

Synonyms

PRP8 homolog, PRP8, Pre-mRNA-processing-splicing factor 8, p220, HPRP8

Validation Data Gallery

Filter:

AllWBIPIHC

WB analysis of Jurkat using 11171-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of K-562 using 11171-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human placenta using 11171-1-AP
human placenta tissue were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 11171-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 11171-1-AP (PRPF8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 11171-1-AP
IP result of anti-PRPF8 (IP:11171-1-AP, 4ug; Detection:11171-1-AP 1:500) with HeLa cells lysate 2000ug.
IHC staining of mouse brain using 11171-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 11171-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human stomach cancer using 11171-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11171-1-AP (PRPF8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Cat No. 11171-1-AP

Size/Concentration

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国内在庫・納期について

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	Jurkat cells, K-562 cells, HeLa cells, human placenta tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	mouse brain tissue, human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 7 publications below
IP	See 1 publications below
RIP	See 1 publications below

Product Information

11171-1-AP targets PRPF8 in WB, IHC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PRPF8 fusion protein Ag1652 相同性解析による交差性が予測される生物種
Full Name	PRP8 pre-mRNA processing factor 8 homolog (S. cerevisiae)
Calculated molecular weight	274 kDa
Observed molecular weight	220 kDa
GenBank accession number	BC064370
Gene symbol	PRPF8
Gene ID (NCBI)	10594
RRID	AB_2171179
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PRPF8, a 220kd protein,is a component of mammalian spliceosomes, large multiprotein complexes involved in the removal of introns from mRNA precursor. It has a central structural role in the architecture of the U5 small nuclear ribonucleoprotein (snRNP) particle.

Protocols

Product Specific Protocols
WB protocol for PRPF8 antibody 11171-1-AP	Download protocol
IHC protocol for PRPF8 antibody 11171-1-AP	Download protocol
IP protocol for PRPF8 antibody 11171-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Mol Cell Mechanism of exon ligation by human spliceosome. Authors - Xiechao Zhan View Article
human	WB	Genes Dev An integrative analysis of colon cancer identifies an essential function for PRPF6 in tumor growth. Authors - Adam S Adler View Article
human	WB	Nucleic Acids Res Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway. Authors - Misbah Khan View Article
human	WB	Nucleic Acids Res XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence. Authors - Shuai Hou View Article
human	WB	RNA Rearrangements within human spliceosomes captured after exon ligation. Authors - Ilagan Janine O JO View Article
human	IP	J Biol Chem Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly. Authors - Zhe Chen View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRPF8 Polyclonal antibody

PRPF8 Polyclonal Antibody for WB, IHC, IP, ELISA

Validation Data Gallery

WB analysis of Jurkat using 11171-1-AP

WB analysis of K-562 using 11171-1-AP

WB analysis of human placenta using 11171-1-AP

WB analysis of HeLa using 11171-1-AP

IP experiment of HeLa using 11171-1-AP

IHC staining of mouse brain using 11171-1-AP

IHC staining of mouse brain using 11171-1-AP

IHC staining of human stomach cancer using 11171-1-AP

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Mechanism of exon ligation by human spliceosome.

An integrative analysis of colon cancer identifies an essential function for PRPF6 in tumor growth.

Sequence-dependent recruitment of SRSF1 and SRSF7 to intronless lncRNA NKILA promotes nuclear export via the TREX/TAP pathway.

XAB2 depletion induces intron retention in POLR2A to impair global transcription and promote cellular senescence.

Rearrangements within human spliceosomes captured after exon ligation.

Identification of a 35S U4/U6.U5 Tri-snRNP Complex Intermediate in Spliceosome Assembly.