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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Peripherin Polyclonal antibody

Peripherin Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Cat no : 17399-1-AP

Synonyms

peripherin, PRPH, NEF4

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Peripherin Polyclonal antibody (17399-1-AP)

    WB analysis using 17399-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 17399-1-AP (Peripherin antibody) at dilution of 1:120000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse colon tissue using Peripherin Polyclonal antibody (17399-1-AP)

    WB analysis of mouse colon using 17399-1-AP

    mouse colon tissue were subjected to SDS PAGE followed by western blot with 17399-1-AP (PRPH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using Peripherin Polyclonal antibody (17399-1-AP)

    WB analysis of COLO 320 using 17399-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17399-1-AP (Peripherin Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human colon tissue using Peripherin Polyclonal antibody (17399-1-AP)

    WB analysis of human colon using 17399-1-AP

    human colon tissue were subjected to SDS PAGE followed by western blot with 17399-1-AP (PRPH antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using Peripherin Polyclonal antibody (17399-1-AP)

    IHC staining of mouse brain using 17399-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17399-1-AP (Peripherin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Peripherin Polyclonal antibody (17399-1-AP)

    IHC staining of human colon using 17399-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17399-1-AP (Peripherin Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon tissue using Peripherin Polyclonal antibody (17399-1-AP)

    IHC staining of human colon using 17399-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 17399-1-AP (Peripherin Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of rat brain tissue using Peripherin Polyclonal antibody (17399-1-AP)

    IHC staining of rat brain using 17399-1-AP

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17399-1-AP (Peripherin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human brain tissue using Peripherin Polyclonal antibody (17399-1-AP)

    IHC staining of human brain using 17399-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17399-1-AP (PRPH antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human brain tissue using Peripherin Polyclonal antibody (17399-1-AP)

    IHC staining of human brain using 17399-1-AP

    Immunohistochemical analysis of paraffin-embedded human brain using 17399-1-AP (PRPH antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of human colon tissue using Peripherin Polyclonal antibody (17399-1-AP)

    IF Staining of human colon using 17399-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Peripherin antibody (17399-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human colon tissue using Peripherin Polyclonal antibody (17399-1-AP)

    IF Staining of human colon using 17399-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human colon tissue using Peripherin antibody (17399-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of PC-12 cells using Peripherin Polyclonal antibody (17399-1-AP)

    FC experiment of PC-12 using 17399-1-AP

    1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Peripherin (17399-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inmouse colon tissue, COLO 320 cells, human colon tissue, rat colon tissue, rat spinal cord tissue
Positive IHC detected inmouse brain tissue, human colon tissue, human brain tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon tissue
Positive FC (Intra) detected inPC-12 cells
Positive FC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

17399-1-AP targets Peripherin in WB, IHC, IF-P, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peripherin fusion protein Ag11365 相同性解析による交差性が予測される生物種
Full Name peripherin
Calculated molecular weight 470 aa, 54 kDa
Observed molecular weight 54-60 kDa
GenBank accession numberBC032703
Gene symbol Peripherin
Gene ID (NCBI) 5630
RRIDAB_2171195
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Peripherin is a neuron-specific intermediate filament protein that is particularly expressed in peripheral nervous system. It is used to study peripheral nerve development and regeneration, as it is highly expressed during elongation of axon. Peripherin is a routine marker for unmyelinated neurons in trigeminal ganglion (TG) and the dorsal root ganglion (DRG). Peripherin is also expressed in peri-insular areas of islets and it has been identified as a key autoantigen for type 1A diabetes.

Protocols

Product Specific Protocols
WB protocol for Peripherin antibody 17399-1-APDownload protocol
IHC protocol for Peripherin antibody 17399-1-APDownload protocol
IF protocol for Peripherin antibody 17399-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIF

Biotechniques

Antibody incubation at 37°C improves fluorescent immunolabeling in free-floating thick tissue sections.

Authors - Xia Xiao
View Article