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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRPH2 Recombinant antibody, PBS Only

PRPH2 Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

242170F4

Cat no : 85043-1-PBS

Synonyms

Peripherin-2, Peripherin2, Peripherin 2, AVMD, AOFMD

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using PRPH2 Recombinant antibody (85043-1-RR)

    WB analysis using 85043-1-RR (same clone as 85043-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85043-1-RR (PRPH2 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PRPH2 Recombinant antibody (85043-1-RR)

    IHC staining of mouse eye using 85043-1-RR (same clone as 85043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85043-1-RR (PRPH2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85043-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse eye tissue using PRPH2 Recombinant antibody (85043-1-RR)

    IHC staining of mouse eye using 85043-1-RR (same clone as 85043-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 85043-1-RR (PRPH2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85043-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PRPH2 Recombinant antibody (85043-1-RR)

    IF Staining of mouse eye using 85043-1-RR (same clone as 85043-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRPH2 antibody (85043-1-RR, Clone: 242170F4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85043-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse eye tissue using PRPH2 Recombinant antibody (85043-1-RR)

    IF Staining of mouse eye using 85043-1-RR (same clone as 85043-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse eye tissue using PRPH2 antibody (85043-1-RR, Clone: 242170F4 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85043-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85043-1-PBS targets PRPH2 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PRPH2 fusion protein Ag12555 相同性解析による交差性が予測される生物種
Full Name peripherin 2 (retinal degeneration, slow)
Calculated molecular weight 346 aa, 39 kDa
Observed molecular weight30-40 kDa
GenBank accession numberBC074720
Gene symbol PRPH2
Gene ID (NCBI) 5961
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Peripherin-2 (PRPH2), also known as retinal degeneration slow protein (RDS), is a photoreceptor-specific tetraspanin protein implicated in outer segment disk morphogenesis. It may function as an adhesion molecule involved in the stabilization and compaction of outer segment disks or in the maintenance of the curvature of the rim. Mutations in peripherin-2 are responsible for various retinal degenerative diseases including autosomal dominant retinitis pigmentosa (ADRP).