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Target

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Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRR5 Polyclonal antibody

PRR5 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 17948-1-AP

Synonyms

Proline-rich protein 5, Proline rich protein 5, proline rich 5 (renal), PP610, FLJ20185k

Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using PRR5 Polyclonal antibody (17948-1-AP)

    WB analysis of human kidney using 17948-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using PRR5 Polyclonal antibody (17948-1-AP)

    WB analysis of HEK-293 using 17948-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PRR5 Polyclonal antibody (17948-1-AP)

    WB analysis of HepG2 using 17948-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using PRR5 Polyclonal antibody (17948-1-AP)

    WB analysis of COLO 320 using 17948-1-AP

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using PRR5 Polyclonal antibody (17948-1-AP)

    WB analysis of mouse brain using 17948-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PRR5 Polyclonal antibody (17948-1-AP)

    WB analysis of human brain using 17948-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 17948-1-AP (PRR5 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PRR5 Polyclonal antibody (17948-1-AP)

    IP experiment of mouse brain using 17948-1-AP

    IP result of anti-PRR5 (IP:17948-1-AP, 3ug; Detection:17948-1-AP 1:300) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PRR5 Polyclonal antibody (17948-1-AP)

    IHC staining of mouse testis using 17948-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PRR5 Polyclonal antibody (17948-1-AP)

    IHC staining of mouse testis using 17948-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PRR5 Polyclonal antibody (17948-1-AP)

    IHC staining of human placenta using 17948-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PRR5 Polyclonal antibody (17948-1-AP)

    IHC staining of human placenta using 17948-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PRR5 Polyclonal antibody (17948-1-AP)

    IHC staining of mouse brain using 17948-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PRR5 Polyclonal antibody (17948-1-AP)

    IHC staining of mouse brain using 17948-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PRR5 Polyclonal antibody (17948-1-AP)

    IHC staining of human placenta using 17948-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PRR5 Polyclonal antibody (17948-1-AP)

    IHC staining of human placenta using 17948-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PRR5 Polyclonal antibody (17948-1-AP)

    IHC staining of mouse brain using 17948-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PRR5 Polyclonal antibody (17948-1-AP)

    IHC staining of mouse brain using 17948-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PRR5 Polyclonal antibody (17948-1-AP)

    IHC staining of mouse kidney using 17948-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using PRR5 Polyclonal antibody (17948-1-AP)

    IHC staining of mouse kidney using 17948-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 17948-1-AP (PRR5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PRR5 Polyclonal antibody (17948-1-AP)

    IHC staining of human kidney using 17948-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17948-1-AP (PRR5 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PRR5 Polyclonal antibody (17948-1-AP)

    IHC staining of human kidney using 17948-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 17948-1-AP (PRR5 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PRR5 Polyclonal antibody (17948-1-AP)

    IF Staining of HepG2 using 17948-1-AP

    Immunofluorescent analysis of HepG2 cells, using PRR5 antibody 17948-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

Positive WB detected inhuman kidney tissue, COLO 320 cells, HEK-293 cells, HepG2 cells, human brain tissue, mouse brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse testis tissue, mouse brain tissue, mouse kidney tissue, human kidney tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IHCSee 1 publications below

Product Information

17948-1-AP targets PRR5 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRR5 fusion protein Ag12424 相同性解析による交差性が予測される生物種
Full Name proline rich 5 (renal)
Calculated molecular weight 31 kDa, 43 kDa
Observed molecular weight 33 kDa, 50 kDa
GenBank accession numberBC016921
Gene symbol PRR5
Gene ID (NCBI) 55615
RRIDAB_2268865
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRR5, also named as PROTOR1 and PP610, is a subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. PRR5 plays an important role in regulation of PDGFRB expression and in modulation of platelet-derived growth factor signaling. It acts as a tumor suppressor in breast cancer. PRR5 has some isoforms with MW 31-33 kDa and 41-50 kDa.

Protocols

Product Specific Protocols
WB protocol for PRR5 antibody 17948-1-APDownload protocol
IHC protocol for PRR5 antibody 17948-1-APDownload protocol
IF protocol for PRR5 antibody 17948-1-APDownload protocol
IP protocol for PRR5 antibody 17948-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Nat Cell Biol

m6A mRNA methylation regulates AKT activity to promote the proliferation and tumorigenicity of endometrial cancer.

Authors - Jun Liu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Tominica (Verified Customer) (08-19-2019)

Rabbit polyclonal anti-FUS/TLS Ab-TLS-M generated against amino acids 260–274 of mouse FUS/TLS, a stretch where the sequence is identical to the human FUS/TLS sequence. works very well.

  • Applications: Western Blot, Immunofluorescence,
  • Cell Tissue Type: human motor neuron