Validation Data Gallery
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Tested Applications
| Positive WB detected in | Raji cells, HepG2 cells, mouse thymus tissue |
| Positive IHC detected in | human small intestine tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 2 publications below |
Product Information
15005-1-AP targets Trypsin 2/PRSS2 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Trypsin 2/PRSS2 fusion protein Ag6461 相同性解析による交差性が予測される生物種 |
| Full Name | protease, serine, 2 (trypsin 2) |
| Calculated molecular weight | 26 kDa |
| Observed molecular weight | 26 kDa, 35 kDa |
| GenBank accession number | BC030260 |
| Gene Symbol | PRSS2 |
| Gene ID (NCBI) | 5645 |
| RRID | AB_2171841 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P07478 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
The human pancreas produces three isoforms of trypsinogen encoded by separate genes, the PRSS1 (protease, serine 1), PRSS2, and PRSS3 genes. PRSS2 is also named as TRY2(trypsin-2), TRYP2. In the ileum, it may be involved in defensin processing, including DEFA5. The preproprotein comprises 247 amino acids, including a 15-amino acid signal peptide and an 8-amino acid activation peptide.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Trypsin 2/PRSS2 antibody 15005-1-AP | Download protocol |
| IHC protocol for Trypsin 2/PRSS2 antibody 15005-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Biol Open Exocrine pancreas proteases regulate β-cell proliferation in zebrafish ciliopathy models and in murine systems. | ||
Tissue Cell PRSS2 overexpression relates to poor prognosis and promotes proliferation, migration and invasion in gastric cancer
| ||
Biochem Biophys Res Commun Epigenome-wide association analysis of pancreatic exocrine cells from high-fat- and normal diet-fed mice and its potential use for understanding the oncogenesis of human pancreatic cancer | ||
Acta Histochem PRSS2 regulates EMT and metastasis via MMP-9 in gastric cancer
|