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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRSS27 Polyclonal antibody

PRSS27 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 19515-1-AP

Synonyms

CAPH2, Marapsin, MPN, Pancreasin, protease, serine 27, PRSS27, Serine protease 27

Validation Data Gallery

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  • Western Blot (WB) analysis of SW 1990 cells using PRSS27 Polyclonal antibody (19515-1-AP)

    WB analysis of SW 1990 using 19515-1-AP

    SW 1990 cells were subjected to SDS PAGE followed by western blot with 19515-1-AP (PRSS27 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of SW 1990 cells using PRSS27 Polyclonal antibody (19515-1-AP)

    IP experiment of SW 1990 using 19515-1-AP

    IP result of anti-PRSS27 (IP:19515-1-AP, 4ug; Detection:19515-1-AP 1:300) with SW 1990 cells lysate 2960ug.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using PRSS27 Polyclonal antibody (19515-1-AP)

    IHC staining of human pancreas using 19515-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 19515-1-AP (PRSS27 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using PRSS27 Polyclonal antibody (19515-1-AP)

    IHC staining of human pancreas using 19515-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 19515-1-AP (PRSS27 Antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using PRSS27 Polyclonal antibody (19515-1-AP)

    IHC staining of human oesophagus cancer using 19515-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 19515-1-AP (PRSS27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human oesophagus cancer tissue using PRSS27 Polyclonal antibody (19515-1-AP)

    IHC staining of human oesophagus cancer using 19515-1-AP

    Immunohistochemical analysis of paraffin-embedded human oesophagus cancer tissue slide using 19515-1-AP (PRSS27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using PRSS27 Polyclonal antibody (19515-1-AP)

    IHC staining of mouse pancreas using 19515-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 19515-1-AP (PRSS27 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse pancreas tissue using PRSS27 Polyclonal antibody (19515-1-AP)

    IHC staining of mouse pancreas using 19515-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 19515-1-AP (PRSS27 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inSW 1990 cells
Positive IP detected inSW 1990 cells
Positive IHC detected inhuman pancreas tissue, human oesophagus cancer tissue, mouse pancreas tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

19515-1-AP targets PRSS27 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protease, serine 27
Calculated molecular weight 32 kDa
Observed molecular weight 28 kDa
GenBank accession numberNM_031948
Gene symbol PRSS27
Gene ID (NCBI) 83886
RRIDAB_2878585
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PRSS27, also named as CAPH2, MPN, belongs to the peptidase S1 family. The trypsin-like serine protease marapsin (Prss27) is a member of the large protease gene cluster at human chromosome 16p13.3. The Prss27 gene is expressed by a variety of human, mouse, and opossum tissues. In humans and mice, it is especially abundant in stratified squamous epithelia of cornea, larynx, esophagus, and cervix and is induced in wounded and psoriatic skin (PMID: 23447538). Prss27 expression was restricted to tissues containing stratified squamous epithelia and was absent or only weakly expressed in all other tissues, including the pancreas.

Protocols

Product Specific Protocols
WB protocol for PRSS27 antibody 19515-1-APDownload protocol
IHC protocol for PRSS27 antibody 19515-1-APDownload protocol
IP protocol for PRSS27 antibody 19515-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols