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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRTFDC1 Polyclonal antibody

PRTFDC1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 11986-1-AP

Synonyms

Phosphoribosyltransferase domain-containing protein 1, HHGP

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using PRTFDC1 Polyclonal antibody (11986-1-AP)

    WB analysis of fetal human brain using 11986-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 11986-1-AP (PRTFDC1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Y79 cells using PRTFDC1 Polyclonal antibody (11986-1-AP)

    WB analysis of Y79 using 11986-1-AP

    Y79 cells were subjected to SDS PAGE followed by western blot with 11986-1-AP (PRTFDC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PRTFDC1 Polyclonal antibody (11986-1-AP)

    WB analysis of human brain using 11986-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 11986-1-AP (PRTFDC1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using PRTFDC1 Polyclonal antibody (11986-1-AP)

    IP experiment of mouse brain using 11986-1-AP

    IP result of anti-PRTFDC1 (IP:11986-1-AP, 4ug; Detection:11986-1-AP 1:600) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using PRTFDC1 Polyclonal antibody (11986-1-AP)

    IHC staining of human ovary tumor using 11986-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor using 11986-1-AP (PRTFDC1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PRTFDC1 Polyclonal antibody (11986-1-AP)

    IF Staining of HeLa using 11986-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 11986-1-AP (PRTFDC1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected infetal human brain tissue, human brain tissue, Y79 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11986-1-AP targets PRTFDC1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRTFDC1 fusion protein Ag2614 相同性解析による交差性が予測される生物種
Full Name phosphoribosyl transferase domain containing 1
Calculated molecular weight 225 aa, 26 kDa
Observed molecular weight 26 kDa
GenBank accession numberBC008662
Gene symbol PRTFDC1
Gene ID (NCBI) 56952
RRIDAB_2284509
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PRTFDC1 antibody 11986-1-APDownload protocol
IHC protocol for PRTFDC1 antibody 11986-1-APDownload protocol
IF protocol for PRTFDC1 antibody 11986-1-APDownload protocol
IP protocol for PRTFDC1 antibody 11986-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

PLoS One

PRTFDC1 is a genetic modifier of HPRT-deficiency in the mouse.

Authors - Keebaugh Alaine C AC
View Article