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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PRTN3 Polyclonal antibody

PRTN3 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 25278-1-AP

Synonyms

ACPA, AGP7, C ANCA, C ANCA antigen, Leukocyte proteinase 3, MBN, MBT, Myeloblastin, Neutrophil proteinase 4, P29, PR 3, PR3, proteinase 3, PRTN3, Wegener autoantigen

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using PRTN3 Polyclonal antibody (25278-1-AP)

    WB analysis of human placenta using 25278-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 25278-1-AP (PRTN3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using PRTN3 Polyclonal antibody (25278-1-AP)

    WB analysis of human placenta using 25278-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 25278-1-AP (PRTN3 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PRTN3 Polyclonal antibody (25278-1-AP)

    IHC staining of human tonsillitis using 25278-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25278-1-AP (PRTN3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using PRTN3 Polyclonal antibody (25278-1-AP)

    IHC staining of human tonsillitis using 25278-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 25278-1-AP (PRTN3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PRTN3 Polyclonal antibody (25278-1-AP)

    IHC staining of human spleen using 25278-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 25278-1-AP (PRTN3 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human spleen tissue using PRTN3 Polyclonal antibody (25278-1-AP)

    IHC staining of human spleen using 25278-1-AP

    Immunohistochemical analysis of paraffin-embedded human spleen tissue slide using 25278-1-AP (PRTN3 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRTN3 Polyclonal antibody (25278-1-AP)

    IHC staining of human colon cancer using 25278-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25278-1-AP (PRTN3 Antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PRTN3 Polyclonal antibody (25278-1-AP)

    IHC staining of human colon cancer using 25278-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 25278-1-AP (PRTN3 Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

Positive WB detected inhuman placenta tissue
Positive IHC detected inhuman tonsillitis tissue, human colon cancer tissue, human spleen tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 25278-1-AP is PRTN3 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PRTN3 fusion protein Ag16765 相同性解析による交差性が予測される生物種
Full Name proteinase 3
Calculated molecular weight 256 aa, 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC096183
Gene symbol PRTN3
Gene ID (NCBI) 5657
RRIDAB_2880003
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PRTN3 antibody 25278-1-APDownload protocol
IHC protocol for PRTN3 antibody 25278-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIHC

Biomedicines

Comparative Proteomic Analysis of tPVAT during Ang II Infusion.

Authors - Xiuying Liang
View Article
humanWB

Front Cell Neurosci

Meningitic Escherichia coli-Induced Interleukin-17A Facilitates Blood-Brain Barrier Disruption via Inhibiting Proteinase 3/Protease-Activated Receptor 2 Axis.

Authors - Bojie Xu
View Article
humanWB

Toxicol In Vitro

Activation of inflammasomes by tyrosine kinase inhibitors of vascular endothelial growth factor receptor: Implications for VEGFR TKIs-induced immune related adverse events.

Authors - Hideki Imano
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Malgorzata (Verified Customer) (08-08-2022)

The antibody worked on my positive control - cell lysate from cultured THP-1 cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1000
  • Cell Tissue Type: THP-1
PRTN3 Antibody Western Blot validation (1000 dilution) in THP-1 (Cat no:25278-1-AP)