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  • KD/KO Validated

PSAP Polyclonal antibody

PSAP Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 18423-1-AP

Synonyms

A1 activator, Cerebroside sulfate activator, Co beta glucosidase, Component C, CSAct, Dispersin, GLBA, Glucosylceramidase activator, Proactivator polypeptide, prosaposin, Protein C, PSAP, SAP 1, SAP 2 Saposin D, SAP1

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSAP Polyclonal antibody (18423-1-AP)

    WB analysis using 18423-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 18423-1-AP (PSAP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PSAP Polyclonal antibody (18423-1-AP)

    WB analysis of HepG2 using 18423-1-AP

    WB result of PSAP antibody (18423-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSAP transfected HepG2 cells.

  • Western Blot (WB) analysis of HeLa cells using PSAP Polyclonal antibody (18423-1-AP)

    WB analysis of HeLa using 18423-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 18423-1-AP (PSAP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using PSAP Polyclonal antibody (18423-1-AP)

    WB analysis of HepG2 using 18423-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18423-1-AP (PSAP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSAP Polyclonal antibody (18423-1-AP)

    IHC staining of human liver cancer using 18423-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18423-1-AP (PSAP antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSAP Polyclonal antibody (18423-1-AP)

    IHC staining of human liver cancer using 18423-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 18423-1-AP (PSAP antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PSAP Polyclonal antibody (18423-1-AP)

    IHC staining of human prostate cancer using 18423-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18423-1-AP (PSAP Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using PSAP Polyclonal antibody (18423-1-AP)

    IHC staining of human prostate cancer using 18423-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 18423-1-AP (PSAP Antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSAP Polyclonal antibody (18423-1-AP)

    IHC staining of human liver cancer using 18423-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18423-1-AP (PSAP Antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSAP Polyclonal antibody (18423-1-AP)

    IHC staining of human liver cancer using 18423-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 18423-1-AP (PSAP Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18423-1-AP targets PSAP in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSAP fusion protein Ag13266 相同性解析による交差性が予測される生物種
Full Name prosaposin
Calculated molecular weight 58 kDa
Observed molecular weight 60-70 kDa
GenBank accession numberBC001503
Gene symbol PSAP
Gene ID (NCBI) 5660
RRIDAB_10643525
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

proteins' (SAPs) that assist in the lysosomal hydrolysis of sphingolipids. After proteolytic processing of the presaposin protein, these 4 released polypeptides are functional activators. Saposin A was encoded by residues 60 to 143 of PSAP, saposin B by 195 to 275, saposin C by 311 to 390, and saposin D by 405 to 487. There are four 12-14 kDa heatstable glycoproteins. This antibody should recognize saposin D.

Protocols

Product Specific Protocols
WB protocol for PSAP antibody 18423-1-APDownload protocol
IHC protocol for PSAP antibody 18423-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols