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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSAPL1 Polyclonal antibody

PSAPL1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 55128-1-AP

Synonyms

Saposin B-Val-like, Saposin B-like, Saposin A-like, PSAP L1, prosaposin like 1

Validation Data Gallery

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  • Western Blot (WB) analysis of multi-cells/tissue using PSAPL1 Polyclonal antibody (55128-1-AP)

    WB analysis of multi-cells/tissue using 55128-1-AP

    WB result of 55128-1-AP.

  • Western Blot (WB) analysis of human testis tissue using PSAPL1 Polyclonal antibody (55128-1-AP)

    WB analysis of human testis using 55128-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 55128-1-AP (PSAPL1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PSAPL1 Polyclonal antibody (55128-1-AP)

    WB analysis of HeLa using 55128-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 55128-1-AP (PSAPL1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PSAPL1 Polyclonal antibody (55128-1-AP)

    IHC staining of human stomach cancer using 55128-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55128-1-AP (PSAPL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using PSAPL1 Polyclonal antibody (55128-1-AP)

    IHC staining of human stomach cancer using 55128-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 55128-1-AP (PSAPL1 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PSAPL1 Polyclonal antibody (55128-1-AP)

    IF Staining of HepG2 using 55128-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 55128-1-AP (PSAPL1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inhuman testis tissue, multi-cells/tissue
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55128-1-AP targets PSAPL1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name prosaposin-like 1
Calculated molecular weight 57 kDa
Observed molecular weight 57-63 kDa
GenBank accession numberNM_001085382
Gene symbol PSAPL1
Gene ID (NCBI) 768239
RRIDAB_10888638
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSAPL1 is a protein like PSAP. It can be cleaved to be 5 chains: Saposin A-like, Saposin B-Val-like, Saposin B-like, Saposin C-like and Saposin D-like. PSAPL1 may activate the lysosomal degradation of sphingolipids. The antibody is specific to PSAPL1.

Protocols

Product Specific Protocols
WB protocol for PSAPL1 antibody 55128-1-APDownload protocol
IHC protocol for PSAPL1 antibody 55128-1-APDownload protocol
IF protocol for PSAPL1 antibody 55128-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols