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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSAT1 Recombinant antibody (81479-1-RR)

    WB analysis using 81479-1-RR (same clone as 81479-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81479-1-RR (PSAT1 antibody) at dilution of 1:12000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81479-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PSAT1 Recombinant antibody (81479-1-RR)

    IF Staining of HepG2 using 81479-1-RR (same clone as 81479-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PSAT1 antibody (81479-1-RR, Clone: 1O22 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 81479-1-PBS in a different storage buffer formulation.

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PSAT1 Recombinant antibody, PBS Only

PSAT1 Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 81479-1-PBS
Clone No.1O22

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Pig

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EPIP, Phosphoserine aminotransferase, PSA, PSAT, PSAT1

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81479-1-PBS targets PSAT1 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Pig samples.

Tested Reactivity Human, Mouse, Pig
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PSAT1 fusion protein Ag0776 相同性解析による交差性が予測される生物種
Full Name phosphoserine aminotransferase 1
Calculated molecular weight 40 kDa
Observed molecular weight 37-40 kDa
GenBank accession numberBC004863
Gene Symbol PSAT1
Gene ID (NCBI) 29968
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y617
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

PSAT1, also named as PSA and PSAT, belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family and SerC subfamily. It catalyzes the reversible conversion of 3-phosphohydroxypyruvate to phosphoserine and of 3-hydroxy-2-oxo-4-phosphonooxybutanoate to phosphohydroxythreonine. PSAT1 represents a new interesting target for CRC therapy. It may be implicated in altered serine metabolism and schizophrenia spectrum conditions. (PMID: 18221502, 20955740)