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Validation Data Gallery

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  • Western Blot (WB) analysis of PC-3 cells using PSCA Polyclonal antibody (17171-1-AP)

    WB analysis of PC-3 using 17171-1-AP (same clone as 17171-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 17171-1-AP (PSCA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17171-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PSCA Polyclonal antibody (17171-1-AP)

    WB analysis using 17171-1-AP (same clone as 17171-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 17171-1-AP (PSCA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17171-1-PBS in a different storage buffer formulation.

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PSCA Polyclonal antibody, PBS Only

PSCA Polyclonal Antibody for WB, Indirect ELISA
Cat No. 17171-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17171-1-PBS targets PSCA in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSCA fusion protein Ag10710 相同性解析による交差性が予測される生物種
Full Name prostate stem cell antigen
Calculated molecular weight 123 aa, 13 kDa
Observed molecular weight 36 kDa
GenBank accession numberBC023582
Gene Symbol PSCA
Gene ID (NCBI) 8000
RRIDAB_2172635
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO43653
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

Prostate stem cell antigen (PSCA) is a protein composed of 123 amino acid residues. PSCA belongs to the LY-6/Thy-1 family of cell surface antigens. It is highly expressed in normal prostate and further up-regulated in prostate cancer, as well as non-prostatic malignancies including gastric cancer. PSCA plays a critical role in cell adhesion, proliferation, and survival. The obasrved MW of PSCA is between 36 to 40 kDa in paper (PMID: 11309275).