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PSD95 Polyclonal antibody

PSD95 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 30255-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, IF, ELISA

Disks large homolog 4, DLG4, FLJ97752, FLJ98574, PSD 95, PSD95, SAP 90, SAP90, Synapse associated protein 90

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration:  150 μL, 450 ug/ml
20 μL, 450 ug/ml
150 μL, 450 ug/ml

¥67,000/ 150 μL

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テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30255-1-AP targets PSD95 in WB, IHC, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSD95 fusion protein Ag33000 相同性解析による交差性が予測される生物種
Full Name discs, large homolog 4 (Drosophila)
Calculated molecular weight81 kDa
Observed molecular weight90-95 kDa
GenBank accession numberNM_001365
Gene Symbol PSD95
Gene ID (NCBI) 1742
RRIDAB_3086283
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP78352
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (disks large homolog 4) gene. PSD-95 is a scaffolding protein of the MAGUK protein family, and engages in several vital protein-protein interactions in the brain with its PDZ domains. It has been suggested that PSD-95 is composed of two supramodules, one of which is the PDZ1-2 tandem domain. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation. Observed MW of PSD95 is 90-95 kDa due to phosphorylation (PMID: 20682303).

Protocols

Product Specific Protocols
WB protocol for PSD95 antibody 30255-1-APDownload protocol
IHC protocol for PSD95 antibody 30255-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

FASEB J

HDAC1/2/3-mediated downregulation of neurogranin is involved in cognitive impairment in offspring exposed to maternal subclinical hypothyroidism

Authors - Xi Yu
humanWB,IF

Biochem Biophys Res Commun

Protective role of TRPV2 in synaptic plasticity through the ERK1/2-CREB-BDNF pathway in chronic unpredictable mild stress rats

Authors - Yitong Zhou
ratWB

Biomedicines

Daytime Dysfunction: Symptoms Associated with Nervous System Disorders Mediated by SIRT1

Authors - Tianke Huang
mouseWB

Environ Pollut

Bisphenol F exposure induces depression-like changes: Roles of the kynurenine metabolic pathway along the "liver-brain" axis

Authors - Zheng Yu
mouseWB

Neurobiol Stress

Effects of autotaxin and lysophosphatidic acid deficiencies on depression-like behaviors in mice exposed to chronic unpredictable mild stress

Authors - Chao Wang
mouseWB

Aging (Albany NY)

Chronic stress induces Alzheimer's disease-like pathologies through DNA damage-Chk1-CIP2A signaling

Authors - Zhuoqun Wang
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