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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSD95-Specific,DLG4 Recombinant antibody, PBS Only

PSD95-Specific,DLG4 Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1I22

Cat no : 81106-1-PBS

Synonyms

Disks large homolog 4, DLG4, FLJ97752, FLJ98574, PSD 95, PSD95, SAP 90, SAP90, Synapse associated protein 90

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSD95-Specific,DLG4 Recombinant antibody (81106-1-RR)

    WB analysis using 81106-1-RR (same clone as 81106-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81106-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PSD95-Specific,DLG4 Recombinant antibody (81106-1-RR)

    IHC staining of mouse brain using 81106-1-RR (same clone as 81106-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81106-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PSD95-Specific,DLG4 Recombinant antibody (81106-1-RR)

    IHC staining of mouse brain using 81106-1-RR (same clone as 81106-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 81106-1-RR (PSD95-Specific,DLG4 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81106-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using PSD95-Specific,DLG4 Recombinant antibody (81106-1-RR)

    IF Staining of mouse brain using 81106-1-RR (same clone as 81106-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PSD95-Specific,DLG4 antibody (81106-1-RR, Clone: 1I22 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81106-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81106-1-PBS targets PSD95-Specific,DLG4 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name discs, large homolog 4 (Drosophila)
Calculated molecular weight 85 kDa
Observed molecular weight90-95 kDa
GenBank accession numberNM_001365
Gene symbol PSD95
Gene ID (NCBI) 1742
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

PSD-95 (postsynaptic density protein 95) also known as SAP-90 (synapse-associated protein 90) is a protein that in humans is encoded by the DLG4 (disks large homolog 4) gene. PSD-95 is a scaffolding protein of the MAGUK protein family, and engages in several vital protein-protein interactions in the brain with its PDZ domains. It has been suggested that PSD-95 is composed of two supramodules, one of which is the PDZ1-2 tandem domain. It plays an important role in synaptic plasticity and the stabilization of synaptic changes during long-term potentiation. Observed MW of PSD95 is 90-95 kDa due to phosphorylation (PMID: 20682303).