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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSEN1 Polyclonal antibody

PSEN1 Polyclonal Antibody for IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 20434-1-AP

Synonyms

AD3, FAD, presenilin 1, Protein S182, PS 1, PS1, PS1 CTF12, PSEN1, PSNL1, S182

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human gliomas tissue using PSEN1 Polyclonal antibody (20434-1-AP)

    IHC staining of human gliomas using 20434-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20434-1-AP (PSEN1 Antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human gliomas tissue using PSEN1 Polyclonal antibody (20434-1-AP)

    IHC staining of human gliomas using 20434-1-AP

    Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 20434-1-AP (PSEN1 Antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PSEN1 Polyclonal antibody (20434-1-AP)

    IHC staining of mouse brain using 20434-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20434-1-AP (PSEN1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse brain tissue using PSEN1 Polyclonal antibody (20434-1-AP)

    IHC staining of mouse brain using 20434-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20434-1-AP (PSEN1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using PSEN1 Polyclonal antibody (20434-1-AP)

    IF Staining of mouse brain using 20434-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PSEN1 antibody (20434-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using PSEN1 Polyclonal antibody (20434-1-AP)

    IF Staining of mouse brain using 20434-1-AP

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using PSEN1 antibody (20434-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using PSEN1 Polyclonal antibody (20434-1-AP)

    IF Staining of HEK-293 using 20434-1-AP

    Immunofluorescent analysis of HEK-293 cells using 20434-1-AP (PSEN1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive IHC detected inhuman gliomas tissue, mouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inmouse brain tissue, HEK-293 cells

Recommended dilution

ApplicationDilution
Immunohistochemistry (IHC)IHC : 1:50-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

20434-1-AP targets PSEN1 in IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSEN1 fusion protein Ag14135 相同性解析による交差性が予測される生物種
Full Name presenilin 1
Calculated molecular weight 467 aa, 53 kDa
Observed molecular weight18 kDa
GenBank accession numberBC011729
Gene symbol PSEN1
Gene ID (NCBI) 5663
RRIDAB_2878685
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSEN1(presenilin-1) is also named as AD3, PS1, PSNL1 and belongs to the peptidase A22A family. It directly influences Netrin/DCC signaling with cell type precision and spatiotemporal specificity, and is a key neural circuit builder that gates the spatiotemporal pattern of guidance signaling, thereby ensuring neural projections occur with high fidelity(PMID:21215373). The 46-kDa PSEN1 holoprotein is cleaved into a ~30-kDa N-terminal fragment (NTF) and a ~20-kDa C-terminal fragment (CTF) by the 26S proteasome(PMID:10527805). It has 7 isoforms produced by alternative splicing. This antibody can recognize all the isoforms except isoform 4 (21 kDa).

Protocols

Product Specific Protocols
IHC protocol for PSEN1 antibody 20434-1-APDownload protocol
IF protocol for PSEN1 antibody 20434-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Front Aging Neurosci

LongShengZhi Capsule Attenuates Alzheimer-Like Pathology in APP/PS1 Double Transgenic Mice by Reducing Neuronal Oxidative Stress and Inflammation.

Authors - Zequn Yin
View Article