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Validation Data Gallery

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  • Western Blot (WB) analysis of human kidney tissue using PSG1 Polyclonal antibody (11809-1-AP)

    WB analysis of human kidney using 11809-1-AP

    human kidney tissue were subjected to SDS PAGE followed by western blot with 11809-1-AP (PSG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using PSG1 Polyclonal antibody (11809-1-AP)

    WB analysis of MCF-7 using 11809-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 11809-1-AP (PSG1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using PSG1 Polyclonal antibody (11809-1-AP)

    IHC staining of human placenta using 11809-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11809-1-AP (PSG1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using PSG1 Polyclonal antibody (11809-1-AP)

    IHC staining of human placenta using 11809-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11809-1-AP (PSG1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using PSG1 Polyclonal antibody (11809-1-AP)

    IF Staining of MCF-7 using 11809-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using PSG1 antibody (11809-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

PSG1 Polyclonal antibody

PSG1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 11809-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

FL NCA 1/2, Fetal liver non-specific cross-reactive antigen 1/2, DHFRP2, CD66f, CD66 antigen-like family member F

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman kidney tissue, MCF-7 cells
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

11809-1-AP targets PSG1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSG1 fusion protein Ag2468 相同性解析による交差性が予測される生物種
Full Name pregnancy specific beta-1-glycoprotein 1
Calculated molecular weight 426 aa, 48 kDa
Observed molecular weight 72 kDa, 36 kDa
GenBank accession numberBC022338
Gene Symbol PSG1
Gene ID (NCBI) 5669
RRIDAB_2171045
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP11464
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PSG1 antibody 11809-1-APDownload protocol
IHC protocol for PSG1 antibody 11809-1-APDownload protocol
IF protocol for PSG1 antibody 11809-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Fertil Steril

Trophoblast retrieval and isolation from the cervix (TRIC) for noninvasive prenatal screening at 5 to 20 weeks of gestation.

Authors - Jay M Bolnick
View Article