Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells |
| Positive IHC detected in | mouse brain tissue, mouse spleen tissue, rat brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 4 publications below |
| IF | See 1 publications below |
| RIP | See 1 publications below |
Product Information
25504-1-AP targets PSIP1 in WB, IHC, IF/ICC, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | PSIP1 fusion protein Ag21886 相同性解析による交差性が予測される生物種 |
| Full Name | PC4 and SFRS1 interacting protein 1 |
| Calculated molecular weight | 530 aa, 60 kDa |
| Observed molecular weight | 75 kDa |
| GenBank accession number | BC064135 |
| Gene Symbol | PSIP1 |
| Gene ID (NCBI) | 11168 |
| RRID | AB_2861312 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75475 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PSIP1 is a chromatin associated protein that is shown to act as a transcriptional coactivator as well as an RNA-binding protein. PSIP1 encodes two protein isoforms with molecular masses of 52 and 75 kDa. p52 and p75 were identified as interacting with transcription factor PC4 and shown to act as transcriptional coactivators.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PSIP1 antibody 25504-1-AP | Download protocol |
| IHC protocol for PSIP1 antibody 25504-1-AP | Download protocol |
| IF protocol for PSIP1 antibody 25504-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Sci Adv LEDGF and HDGF2 relieve the nucleosome-induced barrier to transcription in differentiated cells.
| ||
Elife The Tudor SND1 protein is an m6A RNA reader essential for replication of Kaposi's sarcoma-associated herpesvirus. | ||
Front Immunol Single-cell RNA-seq reveals T cell exhaustion and immune response landscape in osteosarcoma | ||
Cancer Sci N-acetylgalactosaminyltransferase GALNT6 is a potential therapeutic target of clear cell renal cell carcinoma progression |