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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSIP1 Polyclonal antibody

PSIP1 Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 29434-1-AP

Synonyms

CLL associated antigen KW 7, DFS 70, DFS70, LEDGF, p52, p75, PAIP, PSIP1, PSIP2

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSIP1 Polyclonal antibody (29434-1-AP)

    WB analysis using 29434-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29434-1-AP (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PSIP1 Polyclonal antibody (29434-1-AP)

    WB analysis using 29434-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29434-1-AP (PSIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PSIP1 Polyclonal antibody (29434-1-AP)

    IF Staining of HepG2 using 29434-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSIP1 antibody (29434-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Tested Applications

Positive WB detected inHeLa cells, HepG2 cells, MCF-7 cells, Jurkat cells, NIH/3T3 cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29434-1-AP targets PSIP1 in WB, IF/ICC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSIP1 fusion protein Ag30640 相同性解析による交差性が予測される生物種
Full Name PC4 and SFRS1 interacting protein 1
Calculated molecular weight 60 kDa
Observed molecular weight75 kDa
GenBank accession numberNM_021144
Gene symbol PSIP1
Gene ID (NCBI) 11168
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSIP1 enables DNA-binding transcription factor binding activity, chromatin binding activity and transcription coactivator activity. It is involved in mRNA 5'-splice site recognition and positive regulation of transcription by RNA polymerase II.

Protocols

Product Specific Protocols
WB protocol for PSIP1 antibody 29434-1-APDownload protocol
IF protocol for PSIP1 antibody 29434-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols