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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using PSKH2 Polyclonal antibody (18813-1-AP)

    WB analysis of HepG2 using 18813-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 18813-1-AP (PSKH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human brain tissue using PSKH2 Polyclonal antibody (18813-1-AP)

    WB analysis of human brain using 18813-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 18813-1-AP (PSKH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human testis tissue using PSKH2 Polyclonal antibody (18813-1-AP)

    WB analysis of human testis using 18813-1-AP

    human testis tissue were subjected to SDS PAGE followed by western blot with 18813-1-AP (PSKH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human heart tissue using PSKH2 Polyclonal antibody (18813-1-AP)

    WB analysis of human heart using 18813-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 18813-1-AP (PSKH2 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HepG2 cells using PSKH2 Polyclonal antibody (18813-1-AP)

    IP experiment of HepG2 using 18813-1-AP

    IP result of anti-PSKH2 (IP:18813-1-AP, 4ug; Detection:18813-1-AP 1:500) with HepG2 cells lysate 3400ug.

  • Immunohistochemistry (IHC) staining of human kidney tissue using PSKH2 Polyclonal antibody (18813-1-AP)

    IHC staining of human kidney using 18813-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18813-1-AP (PSKH2 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using PSKH2 Polyclonal antibody (18813-1-AP)

    IHC staining of human kidney using 18813-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 18813-1-AP (PSKH2 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PSKH2 Polyclonal antibody (18813-1-AP)

    IF Staining of HepG2 using 18813-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 18813-1-AP (PSKH2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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PSKH2 Polyclonal antibody

PSKH2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Cat No. 18813-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

Serine/threonine-protein kinase H2, PSK-H2, PSK H2, Protein serine kinase H2, EC:2.7.11.1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, human brain tissue, human heart tissue, human testis tissue
Positive IP detected inHepG2 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18813-1-AP targets PSKH2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name protein serine kinase H2
Calculated molecular weight 43 kDa
Observed molecular weight 35 kDa
GenBank accession numberBC126182
Gene Symbol PSKH2
Gene ID (NCBI) 85481
RRIDAB_2171244
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96QS6
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSKH2 belongs to the protein kinase superfamily and CAMK Ser/Thr protein kinase family. PSKH2 catalytic the reaction: ATP + a protein = ADP + a phosphoprotein.

Protocols

Product Specific Protocols
WB protocol for PSKH2 antibody 18813-1-APDownload protocol
IHC protocol for PSKH2 antibody 18813-1-APDownload protocol
IF protocol for PSKH2 antibody 18813-1-APDownload protocol
IP protocol for PSKH2 antibody 18813-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols