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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMA1 Polyclonal antibody

PSMA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 11175-1-AP

Synonyms

PSC2, Proteasome subunit alpha type-1, PROS-30, PROS30, NU

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMA1 Polyclonal antibody (11175-1-AP)

    WB analysis using 11175-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 11175-1-AP (PSMA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using PSMA1 Polyclonal antibody (11175-1-AP)

    IHC staining of human colon cancer using 11175-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer using 11175-1-AP (PSMA1 antibody) at dilution of 1:50 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using PSMA1 Polyclonal antibody (11175-1-AP)

    IF Staining of PC-3 using 11175-1-AP

    Immunofluorescent analysis of PC-3 cells using 11175-1-AP (PSMA1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inCOLO 320 cells, HeLa cells, PC-3 cells, mouse kidney tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

11175-1-AP targets PSMA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMA1 fusion protein Ag1660 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, alpha type, 1
Calculated molecular weight 30 kDa
Observed molecular weight 29-33 kDa
GenBank accession numberBC015105
Gene symbol PSMA1
Gene ID (NCBI) 5682
RRIDAB_2171377
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMA1(Proteasome subunit alpha type-1) is also named as HC2, NU, PROS30, PSC2 and belongs to the peptidase T1A family.The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH.It is induced in breast cancer tissue and up-regulated in liver tumor tissues(PMID:17127214;16317774;17004105).

Protocols

Product Specific Protocols
WB protocol for PSMA1 antibody 11175-1-APDownload protocol
IHC protocol for PSMA1 antibody 11175-1-APDownload protocol
IF protocol for PSMA1 antibody 11175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Int J Mol Sci

Impaired 26S Proteasome Assembly Precedes Neuronal Loss in Mutant UBQLN2 Rats.

Authors - Wenjuan Zhang
View Article