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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMA3 Monoclonal antibody

PSMA3 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A8C9

Cat no : 67938-1-Ig

Synonyms

HC8, Macropain subunit C8, Proteasome component C8, PSC3, PSC8, PSMA3

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMA3 Monoclonal antibody (67938-1-Ig)

    WB analysis using 67938-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67938-1-Ig (PSMA3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMA3 Monoclonal antibody (67938-1-Ig)

    IHC staining of human liver cancer using 67938-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67938-1-Ig (PSMA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMA3 Monoclonal antibody (67938-1-Ig)

    IHC staining of human liver cancer using 67938-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67938-1-Ig (PSMA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA431 cells, U2OS cells, Hela cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67938-1-Ig targets PSMA3 in WB, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PSMA3 fusion protein Ag30552 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, alpha type, 3
Calculated molecular weight 255 aa, 28 kDa
Observed molecular weight28 kDa
GenBank accession numberBC029402
Gene symbol PSMA3
Gene ID (NCBI) 5684
RRIDAB_2918690
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMA3(Proteasome subunit alpha type-3) is also named as HC8, PSC8 and belongs to the peptidase T1A family. The proteasome is a multicatalytic protease complex that catalyzes an energy-dependent, extralysosomal proteolytic pathway responsible for selective elimination of proteins with aberrant structures and naturally occurring short-lived proteins related to metabolic regulation and cell cycle progression. It has 2 isoforms produced by alternative splicing.

Protocols

Product Specific Protocols
WB protocol for PSMA3 antibody 67938-1-IgDownload protocol
IHC protocol for PSMA3 antibody 67938-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols