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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

PSMA4 Monoclonal antibody, PBS Only

PSMA4 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Pig, Rabbit, Rat, Mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E6E4

Cat no : 68203-1-PBS

Synonyms

HC9, HsT17706, Macropain subunit C9, Proteasome component C9, Proteasome subunit L, PSC9, PSMA4

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMA4 Monoclonal antibody (68203-1-Ig)

    WB analysis using 68203-1-Ig (same clone as 68203-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68203-1-Ig (PSMA4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68203-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas cancer tissue using PSMA4 Monoclonal antibody (68203-1-Ig)

    IHC staining of human pancreas cancer using 68203-1-Ig (same clone as 68203-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 68203-1-Ig (PSMA4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68203-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMA4 Monoclonal antibody (68203-1-Ig)

    IF Staining of HeLa using 68203-1-Ig (same clone as 68203-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PSMA4 antibody (68203-1-Ig, Clone: 2E6E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68203-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68203-1-PBS targets PSMA4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Pig, Rabbit, Rat, Mouse samples.

Tested Reactivity Human, Pig, Rabbit, Rat, Mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen PSMA4 fusion protein Ag32626 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, alpha type, 4
Calculated molecular weight 237 aa, 27 kDa
Observed molecular weight29 kDa
GenBank accession numberBC056249
Gene symbol PSMA4
Gene ID (NCBI) 5685
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.