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PSMA7 Monoclonal antibody

PSMA7 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1F2C6

Cat no : 67817-1-Ig

Synonyms

XAPC7, RC6 1, Proteasome subunit RC6-1, Proteasome subunit alpha type-7, HSPC

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMA7 Monoclonal antibody (67817-1-Ig)

    WB analysis using 67817-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67817-1-Ig (PSMA7 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of MCF-7 cells using PSMA7 Monoclonal antibody (67817-1-Ig)

    WB analysis of MCF-7 using 67817-1-Ig

    WB result of PSMA7 antibody (67817-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA7 transfected MCF-7 cells.

  • Western Blot (WB) analysis of HeLa cells using PSMA7 Monoclonal antibody (67817-1-Ig)

    WB analysis of HeLa using 67817-1-Ig

    WB result of PSMA7 antibody (67817-1-Ig; 1:40000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA7 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PSMA7 Monoclonal antibody (67817-1-Ig)

    IHC staining of human cervical cancer using 67817-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67817-1-Ig (PSMA7 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using PSMA7 Monoclonal antibody (67817-1-Ig)

    IHC staining of human cervical cancer using 67817-1-Ig

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67817-1-Ig (PSMA7 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PSMA7 Monoclonal antibody (67817-1-Ig)

    IF Staining of HeLa using 67817-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PSMA7 antibody (67817-1-Ig, Clone: 1F2C6 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of HeLa cells using PSMA7 Monoclonal antibody (67817-1-Ig)

    FC experiment of HeLa using 67817-1-Ig

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug PSMA7 Monoclonal antibody (67817-1-Ig, Clone:1F2C6) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive WB detected inLNCaP cells, MCF-7 cells, HeLa cells, HEK-293 cells, K-562 cells, Jurkat cells, HSC-T6 cells, PC-12 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman cervical cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:1000-1:4000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

67817-1-Ig targets PSMA7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMA7 fusion protein Ag7821 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, alpha type, 7
Calculated molecular weight 28 kDa
Observed molecular weight 28-30 kDa
GenBank accession numberBC004427
Gene symbol PSMA7
Gene ID (NCBI) 5688
RRIDAB_2918580
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMA7(Proteasome subunit alpha type-7) is also named HSPC and belongs to the peptidase T1A family. It is an α-type subunit of the 20S proteasome core complex and participates in degrading proteins through the ubiquitin-proteasome pathway (UPP) which plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis(PMID: 19442227).

Protocols

Product Specific Protocols
WB protocol for PSMA7 antibody 67817-1-IgDownload protocol
IHC protocol for PSMA7 antibody 67817-1-IgDownload protocol
IF protocol for PSMA7 antibody 67817-1-IgDownload protocol
FC protocol for PSMA7 antibody 67817-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Heliyon

PSMA7 promotes the malignant proliferation of esophageal cancer

Authors - Qing-Hua Jiao
View Article