Validation Data Gallery

Filter: AllWBIHCIF/ICCFC (Intra)

WB analysis using 14022-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14022-1-AP (PSMA8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14022-1-AP
WB result of PSMA8 antibody (14022-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA8 transfected HeLa cells.
WB analysis of K-562 using 14022-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 14022-1-AP (PSMA8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse thymus using 14022-1-AP
mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14022-1-AP (PSMA8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human testis using 14022-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14022-1-AP (PSMA8 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 14022-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 14022-1-AP (PSMA8 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse testis using 14022-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14022-1-AP (PSMA8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse testis using 14022-1-AP
Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14022-1-AP (PSMA8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 14022-1-AP
Immunofluorescent analysis of HepG2 cells, using PSMA8 antibody 14022-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
FC experiment of HepG2 using 14022-1-AP
1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PSMA8 Polyclonal antibody (14022-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

PSMA8 Polyclonal antibody

PSMA8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 14022-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

PSMA7L, Proteasome subunit alpha-type 8, Proteasome subunit alpha-type 7-like, Proteasome alpha 4 subunit, Alpha4s

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (3)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	Jurkat cells, K-562 cells, mouse thymus tissue, HeLa cells, rat thymus tissue
Positive IHC detected in	human testis tissue, mouse testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HepG2 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:200-1:500
Immunofluorescence (IF)/ICC	IF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below
IF	See 1 publications below

Product Information

14022-1-AP targets PSMA8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	PSMA8 fusion protein Ag5138 相同性解析による交差性が予測される生物種
Full Name	proteasome (prosome, macropain) subunit, alpha type, 8
Calculated molecular weight	29 kDa
Observed molecular weight	28 kDa
GenBank accession number	BC042820
Gene Symbol	PSMA8
Gene ID (NCBI)	143471
RRID	AB_10896314
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8TAA3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Protocols

Product Specific Protocols
WB protocol for PSMA8 antibody 14022-1-AP	Download protocol
IHC protocol for PSMA8 antibody 14022-1-AP	Download protocol
IF protocol for PSMA8 antibody 14022-1-AP	Download protocol
FC protocol for PSMA8 antibody 14022-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Nucleic Acids Res H3K36me2 methyltransferase NSD2 orchestrates epigenetic reprogramming during spermatogenesis. Authors - Zhiming Li View Article
mouse	IF	PLoS Genet The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility. Authors - Laura Gómez-H View Article
mouse	WB	Sci China Life Sci Homozygous deleterious variants in MYCBPAP induce asthenoteratozoospermia involving abnormal acrosome biogenesis, manchette structure and sperm tail assembly in humans and mice Authors - Yiling Zhou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14022-1-AP

WB analysis of HeLa using 14022-1-AP

WB analysis of K-562 using 14022-1-AP

WB analysis of mouse thymus using 14022-1-AP

IHC staining of human testis using 14022-1-AP

IHC staining of human testis using 14022-1-AP

IHC staining of mouse testis using 14022-1-AP

IHC staining of mouse testis using 14022-1-AP

IF Staining of HepG2 using 14022-1-AP

FC experiment of HepG2 using 14022-1-AP