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  • Featured Product
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PSMA8 Polyclonal antibody

PSMA8 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 14022-1-AP

Synonyms

PSMA7L, Proteasome subunit alpha-type 8, Proteasome subunit alpha-type 7-like, Proteasome alpha 4 subunit, Alpha4s

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMA8 Polyclonal antibody (14022-1-AP)

    WB analysis using 14022-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14022-1-AP (PSMA8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using PSMA8 Polyclonal antibody (14022-1-AP)

    WB analysis of HeLa using 14022-1-AP

    WB result of PSMA8 antibody (14022-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMA8 transfected HeLa cells.

  • Western Blot (WB) analysis of K-562 cells using PSMA8 Polyclonal antibody (14022-1-AP)

    WB analysis of K-562 using 14022-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14022-1-AP (PSMA8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse thymus tissue using PSMA8 Polyclonal antibody (14022-1-AP)

    WB analysis of mouse thymus using 14022-1-AP

    mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14022-1-AP (PSMA8 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human testis tissue using PSMA8 Polyclonal antibody (14022-1-AP)

    IHC staining of human testis using 14022-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14022-1-AP (PSMA8 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using PSMA8 Polyclonal antibody (14022-1-AP)

    IHC staining of human testis using 14022-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 14022-1-AP (PSMA8 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PSMA8 Polyclonal antibody (14022-1-AP)

    IHC staining of mouse testis using 14022-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14022-1-AP (PSMA8 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse testis tissue using PSMA8 Polyclonal antibody (14022-1-AP)

    IHC staining of mouse testis using 14022-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14022-1-AP (PSMA8 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PSMA8 Polyclonal antibody (14022-1-AP)

    IF Staining of HepG2 using 14022-1-AP

    Immunofluorescent analysis of HepG2 cells, using PSMA8 antibody 14022-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HepG2 cells using PSMA8 Polyclonal antibody (14022-1-AP)

    FC experiment of HepG2 using 14022-1-AP

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug PSMA8 Polyclonal antibody (14022-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inJurkat cells, K-562 cells, mouse thymus tissue, HeLa cells, rat thymus tissue
Positive IHC detected inhuman testis tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14022-1-AP targets PSMA8 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PSMA8 fusion protein Ag5138 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, alpha type, 8
Calculated molecular weight 29 kDa
Observed molecular weight28 kDa
GenBank accession numberBC042820
Gene symbol PSMA8
Gene ID (NCBI) 143471
RRIDAB_10896314
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for PSMA8 antibody 14022-1-APDownload protocol
IHC protocol for PSMA8 antibody 14022-1-APDownload protocol
IF protocol for PSMA8 antibody 14022-1-APDownload protocol
FC protocol for PSMA8 antibody 14022-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nucleic Acids Res

H3K36me2 methyltransferase NSD2 orchestrates epigenetic reprogramming during spermatogenesis.

Authors - Zhiming Li
View Article
mouseIF

PLoS Genet

The PSMA8 subunit of the spermatoproteasome is essential for proper meiotic exit and mouse fertility.

Authors - Laura Gómez-H
View Article