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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMB1 Monoclonal antibody (67847-1-Ig)

    WB analysis using 67847-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67847-1-Ig (PSMB1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMB1 Monoclonal antibody (67847-1-Ig)

    IHC staining of human liver cancer using 67847-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67847-1-Ig (PSMB1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using PSMB1 Monoclonal antibody (67847-1-Ig)

    IHC staining of human liver cancer using 67847-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 67847-1-Ig (PSMB1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using PSMB1 Monoclonal antibody (67847-1-Ig)

    IF Staining of U2OS using 67847-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using PSMB1 antibody (67847-1-Ig, Clone: 1C7C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Flow cytometry (FC) experiment of Jurkat cells using PSMB1 Monoclonal antibody (67847-1-Ig)

    FC experiment of Jurkat using 67847-1-Ig

    1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PSMB1 Monoclonal antibody (67847-1-Ig, Clone:1C7C5) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.25 ug Mouse IgG1 isotype control Mouse McAb (66360-1-Ig, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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PSMB1 Monoclonal antibody

PSMB1 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 67847-1-Ig
Clone No.1C7C5

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

1C7C5, FLJ25321, HC5, KIAA1838, Macropain subunit C5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inJurkat cells, K-562 cells, THP-1 cells, U-937 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inU2OS cells
Positive FC (Intra) detected inJurkat cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67847-1-Ig targets PSMB1 in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMB1 fusion protein Ag30791 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, beta type, 1
Calculated molecular weight 241 aa, 26 kDa
Observed molecular weight25 kDa
GenBank accession numberBC020807
Gene Symbol PSMB1
Gene ID (NCBI) 5689
RRIDAB_2918609
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP20618
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMB1(Proteasome subunit beta type-1) is also named as PSC5 and belongs to the peptidase T1B family. The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The gene encodes a 241 amino acid protein with a 28 amino acid propeptide and two glycosylation sites.

Protocols

Product Specific Protocols
WB protocol for PSMB1 antibody 67847-1-IgDownload protocol
IHC protocol for PSMB1 antibody 67847-1-IgDownload protocol
IF protocol for PSMB1 antibody 67847-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols