PSMB11 Polyclonal antibody
PSMB11 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, FC (Intra), ELISA
Conjugate
Unconjugated
Cat no : 55143-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Jurkat cells |
| Positive IHC detected in | human thymus tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:600 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:400 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55143-1-AP targets PSMB11 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Peptide 相同性解析による交差性が予測される生物種 |
| Full Name | proteasome (prosome, macropain) subunit, beta type, 11 |
| Calculated molecular weight | 33 kDa |
| Observed molecular weight | 32-35 kDa |
| GenBank accession number | NM_001099780 |
| Gene symbol | PSMB11 |
| Gene ID (NCBI) | 122706 |
| RRID | AB_10863288 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
PSMB11 belongs to the peptidase T1B family. It is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. PSMB11 has an ATP-dependent proteolytic activity. Incorporated instead of PSMB5 or PSMB8, this unit reduces the chymotrypsin-like activity of the proteasome. It plays a pivotal role in development of CD8-positive T cells. The antibody is specific to PSMB11.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for PSMB11 antibody 55143-1-AP | Download protocol |
| IHC protocol for PSMB11 antibody 55143-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Anja (Verified Customer) (03-16-2022) | We tested the antibody in different dilutions on different cells of the thymus and Psmb11 knockout (KO) mice and could not obtain a specific staining with the antibody. In our hands the signal in the Psmb11-KO mice was comparable to the one obtained from either thymocytes or thymus epithelial cells of Wildtype mice .
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