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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using PSMB2 Monoclonal antibody (68180-1-Ig)

    WB analysis using 68180-1-Ig

    SKOV-3 cells were subjected to SDS PAGE followed by western blot with 68180-1-Ig (PSMB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using PSMB2 Monoclonal antibody (68180-1-Ig)

    WB analysis using 68180-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68180-1-Ig (PSMB2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

  • Western Blot (WB) analysis of HeLa cells using PSMB2 Monoclonal antibody (68180-1-Ig)

    WB analysis of HeLa using 68180-1-Ig

    WB result of PSMB2 antibody (68180-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PSMB2 transfected HeLa cells.

  • Immunohistochemistry (IHC) staining of mouse liver tissue using PSMB2 Monoclonal antibody (68180-1-Ig)

    IHC staining of mouse liver using 68180-1-Ig

    Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 68180-1-Ig (PSMB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using PSMB2 Monoclonal antibody (68180-1-Ig)

    IF Staining of HCT 116 using 68180-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using PSMB2 antibody (68180-1-Ig, Clone: 3C5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using PSMB2 Monoclonal antibody (68180-1-Ig)

    IF Staining of HepG2 using 68180-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using PSMB2 antibody (68180-1-Ig, Clone: 3C5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of PC-3 cells using PSMB2 Monoclonal antibody (68180-1-Ig)

    IF Staining of PC-3 using 68180-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed PC-3 cells using PSMB2 antibody (68180-1-Ig, Clone: 3C5C5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  • Featured Product
  • KD/KO Validated

PSMB2 Monoclonal antibody

PSMB2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 68180-1-Ig
Clone No.3C5C5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

3C5C5, Macropain subunit C7-I, Multicatalytic endopeptidase complex subunit C7-I, Proteasome component C7-I, Proteasome subunit beta type-2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, SKOV-3 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells
Positive IHC detected inmouse liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, HCT 116 cells, PC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68180-1-Ig targets PSMB2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen PSMB2 fusion protein Ag7308 相同性解析による交差性が予測される生物種
Full Name proteasome (prosome, macropain) subunit, beta type, 2
Calculated molecular weight 23 kDa
Observed molecular weight 23 kDa
GenBank accession numberBC000268
Gene Symbol PSMB2
Gene ID (NCBI) 5690
RRIDAB_2935271
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP49721
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PSMB2(proteasome subunit beta type-2) is also named as macropain subunit C7-I, proteasome component C7-I, multicatalytic endopeptidase complex subunit C7-I and belongs to the peptidase T1B family. It is involved in an ATP/ubiquitin-dependent non-lysosomal proteolytic pathway. The up-regulation of PSMB2 may indicate the activated neuronal defensive mechanism in VAD(vitamin A depletion) brain regions, which may underlie the VAD-related psychosis behavior(PMID:21190828).

Protocols

Product Specific Protocols
WB protocol for PSMB2 antibody 68180-1-IgDownload protocol
IHC protocol for PSMB2 antibody 68180-1-IgDownload protocol
IF protocol for PSMB2 antibody 68180-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols